HPLC法同时测定忍冬叶中9种活性成分的研究

Determination of nine active constituents in the leaves of Lonicera japonica Thunb by HPLC

为了有效利用忍冬植株,建立了同时检测忍冬叶中新绿原酸、绿原酸、咖啡酸、芦丁、木犀草苷、金丝桃苷、异绿原酸C、木犀草素和槲皮素9种活性成分含量的HPLC分析方法,并用于忍冬叶中活性成分的定量测定,为忍冬叶多指标质量评价标准提供理论依据和方法参考.

In order to effectively utilize the individual plant of Lonicera japonica Thunb,a method to determine the contents of nine bioactive compounds（ neochlorogenic acid,chlorogenic acid,caffeic acid,rutin,galuteolin,hyperoside,isochlorogenic acid C,luteolin and quercetin） in leaves of Lonicera japonica Thunb was established by HPLC. The method was applied to determine the 9 active ingredients in the leaves. It can apply to evaluate the quality of the leaves of Lonicera japonica Thunb.