ABCB1和ABCC2基因多态性与中国汉族儿童抗结核药物致肝毒性易感性的相关性研究

Association Between Polymorphisms of ABCB1 and ABCC2 Genes and the Susceptibility to Anti-tuberculosis Drug-induced Hepatotoxicity in Chinese Han Children

目的探讨ABCB1和ABCC2基因多态性与中国汉族儿童抗结核药物致肝毒性(anti-tuberculosis drug-induced hepatotoxicity,ATDH)易感性的相关性。方法在中国汉族结核病患儿中,采用病例对照研究,利用高通量的Mass ARRAY平台,对于ABCB1和ABCC2基因的16个标签单核苷酸多态性(single nucleotide polymorphisms,SNPs)位点展开基因分型,并采用logistic回归分析以上SNP位点等位基因和基因型频率在ATDH病例组和ATDH对照组中的分布差异。结果本研究共纳入41例ATDH病例以及189例对照。ABCB1和ABCC2基因SNP位点的等位基因以及基因型在两组间频率分布差异均无统计学意义(P>0.05)。SNP位点分别按照显性遗传模型和隐性遗传模型分析,各位点基因型在两组间频率分布差异均无统计学意义(P>0.05)。结论 ABCB1和ABCC2基因多态性可能与中国汉族儿童ATDH的发生并无相关性。

Objective To investigate the association between polymorphisms of ABCB1 and ABCC2 genes and anti-tuberculosis drug-induced hepatotoxicity（ATDH） in Chinese Han children. Methods A case-control study was performed in Chinese Han pediatric patients with tuberculosis. Mass ARRAY was used for genotyping in 16 tag single nucleotide polymorphisms（SNPs） of ABCB1 and ABCC2 genes. Then,logistic regression was used to analyze differences of allele and genotype distributions of SNP polymorphisms between the case and control groups. Results Totally 41 incident ATDH cases and 189 ATDH-free controls were included in this study. No significant differences were found in the allele and genotype distributions of all SNPs in ABCB1 and ABCC2 genes between the case and control groups（ P〈0. 05）. And there were also no significant differences observed in the genotype distributions of all SNPs in the dominant and recessive heredity models（ P〈0. 05）. Conclusion SNPs of ABCB1 and ABCC2 genes were not associated with ATDH in Chinese Han children.