食用植物油中4种氯丙醇脂肪酸酯的GC-MS测定方法研究

Determination of four kinds of chloropropanols fatty acid esters in edible vegetable oil by GC-MS

采用气相色谱-质谱(GC-MS)联用技术,建立了同时测定食用植物油中3-氯-1,2-丙二醇脂肪酸酯(3-MCPD酯)、2-氯-1,3-丙二醇脂肪酸酯(2-MCPD酯)、1,3-二氯-2-丙二醇脂肪酸酯(1,3-DCP酯)和2,3-二氯-1-丙二醇脂肪酸酯(2,3-DCP酯)含量的分析方法。该方法在0.005~0.5 mg/L范围内线性关系良好,检出限0.1 mg/kg,定量限0.2 mg/kg,加标回收率在97.2%~106.0%之间,相对标准偏差在3.4%~7.1%之间。该方法操作简便,灵敏度高,重复性好,定性定量准确,对仪器污染小,能满足食用植物油中氯丙醇酯的检测需求。

A method for the simultaneous determination of the contents of 3 - monochloropropane - 1,2 - diol （3 - MCPD ester）, 2 - monochloropropane - 1,3 - diol （2 - MCPD ester）, 1,3 - dichloropropane - 2 - ol （ 1, 3 - DCP ester） and 2,3 - dichloropropane - 1 - o1（2,3 - DCP ester） in edible vegetable oil was es- tablished by gas chromatography - mass spectrography （ GC - MS）. The method showed good linear rela- tionship in the range of 0.005 - 0.5 mg/L. The detection limit and quantification limit were 0.1 mg/kg and 0.2 mg/kg respectively. The recovery rate was between 97.2% - 106.0% , and the relative stand- ard deviation was between 3.4% - 7.1%. The method was easily operated with good sensitivity, good repeatability, precise quanlification and quantification and little pollution to instrument, and it could meet the determination demand of chloropropanols fatty acid esters in edible vegetable oil.