Oct-4在ⅢB期结肠癌组织中的表达及与CD45RO^+细胞浸润相关性研究

Expression and clinical significance of Oct-4 in stage ⅢB colon cancer and its correlation with CD45RO^+ cell infiltration

目的观察转录因子Oct-4在ⅢB期结肠癌患者癌组织中的表达,并探讨Oct-4表达水平、部位同CD45RO^+淋巴细胞浸润密度之间的相关性,为结肠癌的诊断和判断预后提供依据。方法选取佳木斯大学附属第一医院65例经外科手术治疗切除的ⅢB期结肠癌患者的癌组织标本、36例癌旁组织标本及24例正常结肠组织标本。采用免疫组织化学染色法检测Oct-4,比较各组组织中Oct-4水平以及分布状况。同时,分析其与CD45RO+淋巴细胞浸润密度的关系。结果(1)Oct-4在结肠癌组织中高表达,在结肠癌旁组织中表达较弱,在正常结肠组织中无表达。(2)Oct-4蛋白表达中、强阳性的结肠癌组织中CD45RO+细胞浸润密度低于Oct-4水平阴性、弱阳性表达的组织。细胞核中表达Oct-4蛋白的结肠癌组织中CD45RO^+T淋巴细胞浸润密度高于核浆共表达的结肠癌组织。结论 Oct-4在ⅢB期结肠癌肿瘤细胞中表达,可作为诊断的参考指标;且Oct-4表达水平的高低可作为判断预后的标准。

Objective To study the expression and clinical significance of POU family of transcription factors Oct-4in stageⅢB colon cancer.Methods 65 ⅢB colon cancer tissue specimens,36para-carcinoma tissue specimens and 24 normal colon tissue specimens were selected.Immunohistochemical staining was conducted to detect the Oct-4,the level and distribution of Oct-4were compared between groups,and the relationship between the Oct-4expression and CD45RO~＋cell infiltration was analyzed.Results（1）Oct-4strongly expressed in stageⅢB colon cancer tissue,weakly expressed in para-carcinoma tissue,while not expressed in normal colon tissue.（2）The density of infiltrated CD45RO~＋cells was higher in the tissue with weak expression of Oct-4than in the tissue with strong expression of Oct-4,and higher in the tissue with Oct-4expressed in nucleus than in the tissue with Oct-4co-expressed in both nucleus and cytoplasm.Conclusion Oct-4has strong expression in stageⅢB colon cancer tissue,which can be used as a diagnostic index,and the level of its expression can be used as a criteria of prognosis.