HPLC法测定葆宫止血颗粒中三七皂苷R_1及人参皂苷Rg_1、Rb_1的含量

Determination of the content of Notoginsenoside R_1,Ginsenoside Rg_1 and Rb_1 in Uterus-Repairing Hemostasis Granules by using HPLC

目的建立测定葆宫止血颗粒中三七皂苷R_1、人参皂苷Rg_1以及人参皂苷Rb_1含量的HPLC法。方法采用安捷伦Eclipse XDB-C_(18)柱(250 mm×4.6 mm),以乙腈-水为流动相梯度洗脱,流速为1.0 ml/min,检测波长为203 nm。结果三七皂苷R_1和人参皂苷Rg_1,Rb_1的线性范围分别为0.277~5.540μg(r=0.9998),0.253~5.060μg(r=0.9998),0.262~5.230μg(r=0.9997)。平均加样回收率分别为89.5%(RSD=2.70%)、91.0%(RSD=1.87%)和90.8%(RSD=1.74%)(n=6)。结论该方法快速简便,专属性强,结果准确,可用于葆宫止血颗粒中三七的质量控制。

Objective To establish the determination of the content of Notoginsenoside R1,Ginsenoside Rg1 and Rb1in Uterus-Repairing Hemostasis Granules by using HPLC.Methods By using the Agilent Eclipse XDB-C（18）column（250 mm×4.6 mm）,the acetonitrile-water was adopted to perform mobile phase gradient elution at flow rate of 1.0 ml/min;and the detection wavelength was 203 nm.Results The linear scope of Notoginsenoside R1,Ginsenoside Rg1 and Rb1ranged from0.277 to 5.540 μg（r=0.9998）,from 0.253 to 5.060 μg（r=0.9998）,from 0.262 to 5.230 μg（r=0.9997）.The average recovery rate was 89.5%（RSD=2.70%）,91.0%（RSD=1.87%） and 90.8%（RSD=1.74%）（n=6）.Conclusion As a simple,reproducible and accurate method,it can be used for quality control of pseudo-ginseng in Uterus-Repairing Hemostasis Granules.