经血宁胶囊凝血作用的药效学研究

Pharmacodynamics study on coagulation function of Jingxuening Capsules

目的探讨经血宁胶囊的凝血作用及其机制,为其临床应用提供依据。方法采用随机数字表法将60只实验小鼠及30只新西兰兔随机分为5组,即空白组、阳性组、经血宁胶囊高剂量组(JXN-H组)、中剂量组(JXNM组)、低剂量组(JXN-L组),连续灌胃给药1周,每天给药1次。末次给药后1 h,测定实验小鼠凝血时间(CT)、新西兰兔凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(FIB)和血浆复钙时间(PRT)。结果经血宁胶囊能显著缩短小鼠凝血时间,JXN-H组、JXN-M组与空白组比较差异有统计学意义(P<0.05);经血宁胶囊各给药组显著缩短家兔PT、APTT、TT、PRT,提高血浆FIB含量,与空白组比较差异有统计学意义(P<0.05或P<0.01)。结论经血宁胶囊具有显著的凝血作用。

Objective To investigate the coagulation function and mechanism of Jingxuening Capsules, and to provide scientific basis for its clinical utility. Methods Sixty experimental mice and 30 New Zealand rabbits were randomly di- vided into five groups by random number table, namely blank control group, positive control group, high dose of Jingxuening Capsules group （JXN-H group）, middle dose group （JXN-M group） and tow dose group （JXN-L group）. Intragastric administration for one weeks, one hour after the last administration, clotting time （CT）, prothrombin time （PT）, activated partial thromboplastin time （APTT）, thromhin time （TT）, fibrinogen （FIB） and plasma reealcification time （PRT） of each group were tested. Results Jingxuening Capsules could significantly shorten the clotting time in mice, compared with blank group, JXN-H group and JXN-M group showed significant differences （P 〈 0.05）. All medication administration groups of Jingxuening Capsules could shorten CT, PT, APTF, TT, PRT in New Zealand rabbits, and increase the content of FIB significantly （P 〈 0.05 or P 〈 0.01） compared with the blank group. Conclusion Jingxuening Capsules have marked coagulation function in vivo.