经方青娥丸对去势大鼠骨质疏松作用机制的实验研究

Experimental study on the effect of Qing E Formula on postmenopausal osteoporosis in rats induced by ovariectomy

目的探讨经方青娥丸治疗雌性大鼠卵巢切除后所致骨质疏松的作用机制。方法采用卵巢切除术建立绝经后骨质疏松大鼠模型,建模8周后,分别进行药物干预12周后,双能X线法测定各组大鼠的骨密度、骨组织形态计量学测定骨微结构变化、ELISA检测血清中骨代谢标志物BALP、CTX-1表达情况、以及雌激素E2水平变化。结果 1.骨密度:与治疗前比较,OVX组股骨及椎骨骨密度值均显著降低;雌激素组治疗后股骨和椎骨骨密度值较治疗前均有明显升高;青娥丸组治疗后股骨和椎骨骨密度值也均有一定程度升高。2.骨组织形态计量学:与OVX组比较,QEW组、E2组TBV%均显著升高,TRS%、TFS%、MAR、m AR和OSW均显著降低。与SHAM组比较,QEW组和E2组TBV%均显著降低,TRS%、TFS%均显著增高;QEW组MAR显著增高,E2组则无明显差异;E2组m AR和OSW均显著增高,而QEW组则无明显差异。3.血清骨代谢生化标志物水平:E2组和QEW组BALP水平较OVX组均显著升高、CTX水平均显著降低,QEW组CTX水平显著高于E2组。4.雌激素水平:E2组和QEW组雌激素水平较OVX组均显著升高,而QEW组雌激素水平显著低于E2组。结论青娥丸治疗绝经后大鼠骨质疏松症可能与改善骨微结构、调节骨代谢、雌激素样作用相关。

Objective The current study aimed to explore the possible mechanisms that could contribute to the effects of Qing’ E formula on postmenopausal osteoporosis（PMOP） by establishing a mouse model featuring PMOP.Methods Postmenopausal osteoporosis was established by using ovariectomyinduced rat model.8 weeks after modeling,each group received drug therapy for12 weeks.Dual X-ray absorptiometry was used to measure bone mineral density,bone tissue morphology metrology to determine the bone micro structure changes,ELISA to detect the bone metabolic markers,BALP and CTX-L,expression levels and estrogen E2 level changes in serum.Results 1.BMD：compared with before the treatment,the lumbar spine and femur BMD values decreased significantly in the OVX group,increased significantly in the E2 group,and increased but did not reach significance level in the Qing’E Formula group.2.Bone histomorphometry parameters：The TBV%in E2 group and QEW group were significantly higher and TRS%,TFS%,MAR,mAR and OSW were significantly lower than those of the OVX group.The TBV%in E2 group and QEW group were significantly lower and TRS%and TFS%significantly higher than those of the SHAM group.MAR in QEW group were significantly higher than that of SHAM group,but not significantly from that of the E2 group.mAR and OSW in E2 group were significantly higher than that of SHAM group,but not significantly different from that of the QEW group.3.Serum bone metabolism markers：The level of BALP in E2 group and QEW group were significantly higher and CTX were significantly lower than those of the OVX group.The level of CTX in QEW group were significantly higher than that of the E2 group.4.Estrogen level：The level of estrogen in E2 group and QEW group were significantly higher than that of the OVX group,but the level of estrogen in QEW group were significantly lower than that of the E2 group.Conclusion Qing’E formula can treat PMOP,which may be associated with improving bone microarchitecture,regulating bone metabolism,and estrogenic effects.