补肾、健脾、活血法对去卵巢致骨质疏松症大鼠骨骼、骨骼肌IкBα含量影响的对比研究

Effect of tonifying kidney,invigorating the spleen,and promoting blood circulation on the protein expression of IκBα in bone and skeletal muscle tissues of the rats with osteoporosis

目的观察补肾、健脾、活血法对去卵巢致骨质疏松症大鼠骨骼、骨骼肌IкBα含量变化的影响,探讨中医防治骨质疏松症的作用机制。方法将SD雌性大鼠随机分为正常组、模型组、补肾组、健脾组、活血组。采用酶联免疫吸附(ELISA)法测定大鼠骨骼、骨骼肌IкBα含量变化。结果 1与正常组比较,模型组大鼠骨骼的IкBα含量显著降低(P<0.01);与模型组比较,补肾组、健脾组、活血组大鼠骨骼的IкBα含量明显升高(P<0.01),其中补肾组升高程度比较明显,其次为健脾组。2与正常组比较,模型组大鼠骨骼肌的IкBα含量显著降低(P<0.01);与模型组比较,补肾组、健脾组、活血组大鼠骨骼肌的IкBα含量均有升高,但与模型组比较无统计学意义。结论 1骨质疏松症大鼠骨骼、骨骼肌的IкBα含量均降低,提示骨质疏松症发生可能与骨骼、骨骼肌协调性下降有关。2补肾法和健脾法提高骨质疏松症大鼠的骨骼、骨骼肌IкBα含量,对骨质疏松症具有一定的防治作用。3补肾法是防治骨质疏松症的基本治法,健脾法是辅助补肾法治疗骨质疏松症的一个重要治法。

Objective To observe the effect of tonifying kidney,invigorating the spleen,and promoting blood circulation on the protein expression of IκBα in bone and skeletal muscle tissues of the rats with ovariectomy-induced osteoporosis,and to explored the mechanism of TCM in the prevention and treatment of osteoporosis.Methods Rats were randomly divided into normal control group,model control group,tonifying kidney group,invigorating the spleen group,and promoting blood circulation group.The protein expression of IκBα was determined with ELISA method.Results（1） Compared with that in normal control group,the content of IκBα enzyme in bone tissues of model control group decreased（P 〈0.01）.Compared with that in model control group,the content of IκBα enzyme in bone tissues of tonifying kidney group and invigorating the spleen group increased.（2） Compared with that in normal control group,the content of IκBα enzyme in skeletal muscle tissues of model control group decreased（P 〈0.01）,and the content of IκBαenzyme in skeletal muscle tissues of tonifying kidney group and invigorating the spleen group increased,but there was no statistics difference compared with model control group.Conclusion（1） IκBα decreases in both bone and skeletal muscle in osteoporosis rats,indication that osteoporosis may be related to the disharmony of bone and skeletal muscle.（2） The method of tonifying kidney and invigorating the spleen have certain preventive effect on osteoporosis by regulating the protein expression of IκBα in bone and skeletal muscle.（3） The method of tonifying kidney is fundamental in prevention and treatment of osteoporosis,while the method of invigorating the spleen is also an important complimentary treatment.