“益阳坚阴”中药复方干预豚鼠形觉剥夺性近视实验研究:眼球活体参数测量

Studies on Yiyangjianyin compound prescription in intervening guinea pig form deprivation myopia: eye living parameters measurements

目的观察"益阳坚阴"中药复方对豚鼠实验性近视眼球活体参数的影响。设计实验性研究。研究对象50只花豚鼠。方法随机分为正常对照组(A组)、空白对照组(B组)、"益阳坚阴"中药干预组(C组)、"养阴"中药干预组(D组)。A组双眼无缝合,B、C、D三组缝合豚鼠右眼,诱导建立形觉剥夺性近视模型;B组无药物干预,C组"益阳坚阴"中药复方干预(代水饮),D组养阴中药干预(代水饮)。实验前及实验6周后A超测量眼球活体参数指标。主要指标玻璃体腔深度、眼轴长度。结果各组豚鼠右眼(缝合眼)比较,C组玻璃体腔深度(3.262±0.208)mm、眼轴(7.687±0.256)mm,明显低于B组(3.499±0.098)mm、(8.049±0.257)mm(P均<0.05);各组豚鼠右眼玻璃体腔深度、眼轴长度两两比较发现,A组与C组比较差异无统计学意义(P均>0.05),表明C组在右眼睑缝合下,剥夺性近视眼的发生不明显。组内对比:C组左、右眼眼轴长度(7.664±0.076 mm,7.687±0.256 mm)比较无统计学意义(P>0.05)。结论"益阳坚阴"中药复方可抑制豚鼠形觉剥夺性近视眼的发展,主要表现在延缓了玻璃体腔的加深和眼轴的增长。

Objective To observe the effect of Yiyangjianyin compound prescription on eye living parameters of guinea pig form deprivation myopia. Design Experimental study. Participants 50 guinea pigs. Method Pigs were divided randomly into normal control group （group A）, blank control group （group B）, Yiyangjianyin compound prescription intervention group （group C）, simple Yangyin compound prescription intervention group （group D）. Both eyes of guinea pigs were not stitched up in group A, right eyes of guinea pigs were stitched up in group B, C, D to form deprived myopia model. There was no medicine intervention in Group B. Yiyangjianyin com- pound prescription intervention instead of water dinking was performed in Group C, and simple Yangyin prescription intervention in- stead of water drinking in Group D. Type-A ultrasonic was examined to measure eyeball parameters before experiments and after 6 weeks. Main Outcome Measures Vitreous chamber depth, axial length. Result The vitreous chamber depth （3.262±0.208） mm, axial length （7.687±0.256 mm） in Group C was significantly shorter than that of Group B （3.499±0.098） mm and （8.049±0.257） mm. The dif- ferences was statistical significance （all P〈0.05）. Vitreous chamber depth, axial length of right eye in four groups were compared in pairs. The difference was not statistical significance between Group A and C （all P〉0.05）, which showed the occurrence of deprivation myopia was not obvious in group C with the right eyelid suture. The differences of axial length was not statistical significance between right and left eyes within group C （P〉0.05）. Conclusions Yiyangjianyin compound prescription can in some degree inhibit the development of guinea pig form deprivation myopia, which mainly delayed the deepening of vitreous chamber and lengthening of ocular axis.