氟西汀联合对侧眼遮盖对成年大鼠弱视的治疗作用

The effects of fluoxetine combining covering of the nonamblyopic eye on the treatment of amblyopia in the adult amblyopic rat

目的研究氟西汀(fluoxetine,FLx)口服配合传统遮盖治疗对成年大鼠弱视的治疗效果。设计实验研究。研究对象成年弱视大鼠(P70)。方法制作大鼠成年弱视模型,根据干预方式不同将弱视大鼠随机分为对照组(正常饮水喂养)、Flx2组(20%FLx水喂养2周)、Flx4组(20%FLx水喂养4周)、BFD组(双眼形觉剥夺2周),各组大鼠行传统非弱视眼遮盖(翻转缝合眼)治疗后行图形视觉诱发电位(PVEP)检查。主要指标PVEP振幅,对侧眼与同侧眼振幅比值(the ratio of contralateral versus ipsilateral,C/I比值)及组间比较的HSD-Q值。结果 Flx4组及BFD组配合非弱视眼遮盖治疗1周后弱视眼PVEP振幅由(19.86±3.07)μv、(17.51±4.12)μv升高至(32.31±4.29)μv、(29.31±2.29)μv(t=-4.938、-4.301,P=0.001、0.001);C/I值由1.11±0.29、1.04±0.28提高至2.34±0.45、2.45±0.29(t=-2.743、-3.629,P=0.016、0.007);遮盖治疗后C/I值组间差异显著(F=18.86,P=0.018;对照组与Flx2组:HSD-Q=0.13,P=0.175;Flx2组与Flx4组:HSD-Q=9.76,P=0.013;Flx4组与BFD组:HSD-Q=0.09,P=0.350);BFD组还出现健眼PVEP振幅下降(t=2.672,P=0.047)。FLx2组及对照组弱视眼PVEP振幅无明显提高,C/I比值未出现明显变化。结论20%氟西汀水溶液口服4周与双眼形觉剥夺2周均可稳定激活成年弱视大鼠视皮层可塑性;氟西汀口服配合非弱视眼遮盖治疗可成为成年大鼠弱视的治疗手段。

Objective To investigate the therapeutic effect of fluoxetine on the treatment of amblyopia in the amblyopic adult rat. Design Experimental study. Participants Amblyopic adult rats. Methods The rats were grouped to control, Flx2, Flx4, BFD up on dif- ferent intervention. The rats of control group were dfinked conventional water for 4 weeks. The rats of Flx2 and Flx4 group were drinked 20% fluoxetine drinking water for 2 and 4 weeks. Binocular form deprivation was performed on the rats of BFD group for 2 weeks. Pat- tern visual evoked potential （PVEPs） were measured in each group after 1 week of covering of the nonamblyopic eye. Main Outcome Measures The amplitude of PVEP, the C/I VEP ratio and the HSD-Q. Results There was a increased response of the amblyopic eye （Flx4 group： from19.86±3.07 to 32.31±4.29 μv, Paired t test, t= -4.938, P=0.001. BFD group： from 17.51±4.12 to 29.31±2.29 μv, Paired t test, t=-4.301 ,P=0.001） and the C/I VEP ratio was changed in the rats of Flx4 （from 1.11±0.29 to 2.34±0.45, Paired t test, t= -2.743, P=0.016）and BFD （from 1.04±0.28 to 2.45±0.29, Paired t test, t=-3.629, P=0.007）group. There was statistically significant differience among the C/I VEP ratio of different interventions （F= 18.86, P=0.018; Control vs Flx2： HSD-Q = 0.13, P=0.175; Flx2 vs Flx4： HSD-Q=9.76, P=0.013; Flx4 vs BFD： HSD-Q = 0.09, P=0.350）. A reduction of the response to stimulation of the nonamblyopic eye （Paired t test,t=2.672, P=0.047）was also performed in the BFD group. In contrast, one week of covering in the control group and Flx2 group did not change binocularity in the visual cortex of the amblyopic adult rat. Conclusion Four weeks of 20% fluoxetine drinking and 2 weeks of BFD may restore visual cortex plasticity in the amblyopic adult rat. Chronic administration of fluoxetine can promote the recovery of visual functions in adult amblyopic rats as tested electrophysiologically and may become a new therapy of adult amblyopia.