PTPN13在人胃癌组织和胃癌SGC-7901细胞系中的表达及其对细胞增殖和侵袭的影响

Expression of PTPN13 in human gastric cancer and gastric cancer SGC-7901 cell line and its effect on cell proliferation and invasion

目的分析PTPN13在人胃癌组织中及胃癌SGC-7901细胞系中的表达及其对细胞增殖、侵袭的影响。方法收集106例胃癌患者的胃癌组织及癌周正常组织标本。常规培养SGC-7901细胞并分为pc DNA3.1-PTPN13质粒转染组和未转染组。免疫组织化学法检测组织中的PTPN13表达;分析PTPN13表达与患者性别、年龄、肿瘤部位、肿瘤大小、浸润深度及转移的关系;Kaplan-Meier曲线分析不同PTPN13表达患者生存率的差异;CCK-8法检测细胞的增殖能力;Trans-well试验分析侵袭能力;Western blot检测E-cadherin、Snail及MMP9的表达。结果胃癌组织中的PTPN13阳性率低于癌周正常胃组织(31%vs 83%,P<0.05);PTPN13的表达与肿瘤直径、浸润深度、淋巴结和远隔器官转移情况有关(P<0.05);PTPN13阴性的胃癌患者2生存率较低;PTPN13过表达可以降低SGC-7901细胞的增殖率(P<0.05),同时降低其侵袭能力(P<0.05);上调PTPN13后SGC-7901细胞上皮化标志物E-cadherin表达增加,而间质化标志物Snail和MMP9表达减少。结论 PTPN13在胃癌组织和胃癌细胞中具有肿瘤抑制作用,较低的PTPN13表达提示患者预后不良。PTPN13具有成为胃癌的治疗诊断或治疗靶点的潜力。

Objective To investigate the expression of PTPN13 in human gastric cancer and gastric cancer SGC-7901 cell line and its association with proliferation and invasion. Methods 106 cases gastric cancer tissue samples and matched normal peritumorial tissues were collected. SGC-7901 cells were cultured and divided into two groups including pc DNA3. 1-PTPN13 transfection group and without transfection group. Immunohistochemical technique was used to detecte protein expression of PTPN13. The association of PTPN13 expression with tumor location,tumor size,depth of invasion and tumor metastasis were analyzed. The survival rate of patients with different PTPN13 expression was calculated by Kaplan-Meier curves. CCK-8 assay was used to estimate the proliferation changes. Using Trans-well assay analyzed the invasion of SGC-7901 cells. Moreover,Western blot was performed to detect the markers of EMT including E-cadherin,Snail and MMP9. Results Immunohistochemistry showed the expression rate of PTPN13 in gastric cancer tissues was lower than normal tissue（ 31 % vs 83%,P 0. 05）. Theexpression of PTPN13 in patients was related to with different tumor size,depth of invasion and tumor metastasis（ P 0. 05）. The 2-year survival rate of patients with negative PTPN13 expression were lower. Overexpression reduced both proliferation and invasion in SGC-7901 cells. Up-regulated PTPN13 may increase E-cadherin level but decreases the level of Snail and MMP9. Conclusions PTPN13 plays a role in gastric cancer tissue and cells as a tumor suppressor. Lower PTPN13 may predict a poor prognosis. PTPN13 may be used as therapy target in gastric cancer.