长链非编码RNA在强直性脊柱炎患者中的差异表达研究

LncRNAs expression profile of ankylosing sporidylitis revealed by microarray

目的分析长链非编码RNA（lncRNAs）在强直性脊柱炎患者中的表达情况，探讨1n—cRNAs在强直性脊柱炎发病中的分子机制。方法采用lncRNAs表达谱芯片技术，检测强直性脊柱炎患者和健康体检者外周血单个核细胞中的差异表达lncRNAs和mRNAs，对差异表达的基因进行GO及Pathway分析，并运用Realtime—PCR技术对差异表达的lncRNAs进行验证。结果芯片数据结果获得了具有2倍以上差异表达的lncRNAs共148条，差异表达的mRNAs有134条。GO分析发现，差异表达的基因主要参与蛋白结合、转录调节、代谢、信号转导等。Pathway分析显示它们主要参与Toll样受体、蛋白激酶、补体途径及Notch信号通路等。Realtime—PCR检测了3个lncRNAs在强直性脊柱炎患者中的表达与基因芯片一致，其中D90064是表达差异最为明显的lncRNAs。结论多条lncRNAs在强直性脊柱炎患者外周血单个核细胞中异常表达，提示它们在强直性脊柱炎发生、发展过程中可能发挥着重要作用。

Objective To investigate the expression profile variation of long non-coding RNAs （ lncRNAs） in ankylosing sporidylitis （AS） and explore the role of lncRNAs in the pathogenesis of AS. Methods The peripheral blood mononuclear cells of AS patients and health controls （HC） were used to detect for differently expressed lneRNAs by microaIray. The roles of lncRNAs were predicted with GO and pathway analysis. The results were verified by real time-polymerase chain reaction （PCR）. Results A total of 148 lncRNAs and 134 mRNAs were detected, which had more than 2-fold differentially expressed in AS patients. Bioinformatics analysis found that GO term enrichment included protein binding, regulation of transcription, metabolism, signal transduction, et ah and might involve in toll-like receptor pathway, protein kinase, complement pathway, notch signaling pathway and so on. The expressions of three lncRNAs were estimated by real time-PCR which found that consistent with that of microarrays. Among these, D90064 was the most aberrantly expressed lncRNAs. Conclusions Several lncRNAs expression was changed significan-tly in AS patients in comparison with HC, which implies that those different lncRNAs may have an important role in the development and progression of AS.