高山羊齿孢子的组织培养

Tissue Culture of Spores of Davallia mariesii Moore ex Bak

[目的]探讨不同灭菌方式对组织培养中高山羊齿孢子成活率的影响以及不同培养基对孢子萌发率的影响。[方法]利用NaClO、NaClO+酒精、升汞、升汞+酒精、升汞+NaClO、酒精这6种不同灭菌方式研究其对高山羊齿孢子成活率的影响;考虑4因素基本培养基、蔗糖、NAA、6-BA进行L_(16)(4~4)正交设计,研究其对高山羊齿孢子萌发率的影响。[结果]6种灭菌处理中,NaClO+酒精的灭菌效果最好;酒精的灭菌效果最差;在正交实验中,以1/2MS+3%蔗糖+1mg/L 6-BA+0.1mg/L NAA为高山羊齿孢子萌发的最佳培养基,萌发率达63.69%,以不加任何激素的MS培养基中萌发率最低。[结论]2种不同的灭菌剂混合使用比单独使用灭菌剂的效果好;基本培养基对高山羊齿孢子萌发率的影响最大,影响顺序为:基本培养基>6-BA>蔗糖>NAA。

[Purpose]Discussing the influence of the survival rate and germination rate of spores of Davallia mariesii Moore ex Bak in the different sterilization ways.[Methods]Use the six different sterilization ways which are NaC10,NaC10 add alcohol, mercuric chloride, mercuric chloride add alco- hol,mercuric chloride add NaClO,alcohol to reseach the influence of the survival rate of spores of Davallia mariesii Moore ex Bak;Consider the four factors which are the minimum medium,sucrose, NAA,6-BA to make the L16 （44 ） orthogonal design, study the influence of the germination rate of spores of Davallia mariesii Moore ex Bak.[Result] In the six different sterilization ways, the best sterilization effect is NaC10 add alcohol and the worst is alcohol. In the orthogonal experiment, the best germination medium of the Davallia mariesii Moore ex Bak spores is 1/2 MS＋3% sucrose＋ 1 mg/L 6-BA＋ 0.1 mg/L NAA, the germination rate is 63.6 9 %, the lowest germination rate medium is MS which is add no hormone. [Conclusion] the effect which is two sterilization mixed use is better to exclusive use;The greatest impact for the germination rate of spores of Davallia mariesii Moore ex Bak is minimum medium, the influence order is minimum medium, 6-BA, sucrose, NAA.