UPLC-ELSD法测定人参中果糖、葡萄糖、蔗糖、麦芽糖含量

UPLC-ELSD Simultaneous Determination of Fructose,Glucose,Sucrose and Maltose in Ginseng

建立超高效液相色谱-蒸发光散射法,同时测定人参中果糖、葡萄糖、蔗糖、麦芽糖的含量。色谱柱:ACQUITYUPLCBEHAmide(2.1mm 100mm,1.7 m);流动相:0.2%三乙胺乙腈溶液和水梯度洗脱;流速:0.2m L/min;检测器:ELSD;漂移管温度:75℃;氮气压:30psi。果糖、葡萄糖、蔗糖和麦芽糖在0.1mg/m L^0.8mg/m L内有良好的线性关系,精密度RSD<5.0%,回收率>96.0%。本方法在7min内完成了4种糖的分离,结果比较理想,可作为人参中小分子糖的质量控制和安全评价的测定方法。

A method for the simultaneous determination of 4 sugars such as fructose,glucose,sucrose and maltose in ginseng is described,in which ultra performance liquid chromatography and evaporative light scattering detectionare used. A acquity uplc BEH Amide（2.1mm 100 mm,1.7 m）column kept at 35℃ and eluted with 0.2% TEA acetonitrile solution and aqueous at a flow rate of 0.2m L/min was used for the chromatographic separation.The detector drift tube was kept at 75℃ and the nitrogen pressure was set at 30 psi. The response of the detector to analyte concentration over the range from 0.1mg/m L to 0.8mg/m L displayed good linearity. Precision RSDs of6 replicates for the determination of the sugars were less than 5.0% and mean recoveries for the sugars in 6 real samples were more than96.0%. A good separation among the analytes and other components was achieved within 7 min.This method is accurate and good reproducibility suitable for the measurement of four sugars in the roots of ginseng.