软枣猕猴桃原生质体酶解液配方筛选

Screening of Enzymatic Hydrolysate Formula of Actinidia arguta Protoplast

[目的]筛选软枣猕猴桃原生质体最佳酶解液配方。[方法]以4种配方酶解液在相同条件下处理等量软枣猕猴桃叶片,比较最后收集的纯化原生质体量,筛选最佳酶解液配方。[结果]配方Ⅲ(1.0%纤维素酶、0.5%离析酶、0.3%果胶酶)和Ⅳ(1.0%纤维素酶、0.5%果胶酶)处理酶解效果要明显优于配方Ⅰ(2.0%纤维素酶、0.5%离析酶)、Ⅱ(2.0%纤维素酶、0.3%果胶酶)。[结论]配方Ⅲ(1.0%纤维素酶、0.5%离析酶、0.3%果胶酶)为软枣猕猴桃原生质体最佳酶解液配方。

[ Objective] To screen the best enzymatic hydrolysate formula of Actinidia arguta protoplast. [ Method ] The leaves of A. arguta were treated with four enzymatic hydrolysate formula under the same conditions. The purified protoplast was collected and compared to screen the optimal enzymatic hydrolysate formula. [ Result] The amount of protoplast collected by the solution of formula m （ 1.0% cellulase, 0.5% segregation enzyme and 0.3% pectinase） and formula Ⅳ （ 1.0% cellulase ,0.5% pectinase）were significantly higher than the solution of formula Ⅰ （2.0% cellulase, O. 5% segregation enzyme） and formula Ⅱ （2.0% cellulase, O. 3% pectinase）. [ Conclusion] Formula m （ 1.0% cellulase, 0. 5% segregation enzyme and 0.3% pectinase） is the best enzymatic hydrolysate formula for A. arguta protoplast.