摘要
建立用于测定菇茑果实中叶黄素含量的检测方法。方法以正己烷为溶剂,采用浸泡法提取样品中的叶黄素,NaOH-CH_3OH溶液皂化后利用HPLC测定其含量。色谱柱为Kromassil C_(18)柱(5μm,250 mm×4.6 mm),流动相为甲醇,流速为1.0 mL/min,检测波长为445nm。结果叶黄素在2.3~36.8μg/mL范围内呈良好的线性关系。结论该方法准确度高、重现性好,可适于菇茑果实中叶黄素含量的定量分析。
To establish the method of determination for Lutein in Physali alkekengi L.. Lutein was extrated by soaking methordwith hexane as solvent, and was aponified by NaOH -CH3OH, and the content was determined by HPLC with methanol asmobile phase at the flow of 1.0 mL/min-l, Kromasil CIS as Column, 445 nm as the detection wavelength. The linear relationshipof Lutein was 2.3-36.8μg/mL. This method was accurate and steady, and can be used for the determination of Lutein inPhysali alkekengi L.
作者
苟晓亮
刁全平
郭华
刘雍贵
王晓慧
Gou Xiaoliang Diao Quanping Guo Hua Liu Yonggui Wang Xiaohui(College of Chemistry and Life Science, Anshan Normal University, Anshan 114007, China)
出处
《山东化工》
CAS
2017年第5期67-68,共2页
Shandong Chemical Industry
基金
辽宁省大学生创新创业训练计划项目(2015101690015)
关键词
菇茑
叶黄素
HPLC
Physali alkekengi L.
Lutein
HPLC
