橄榄油加工液体废弃物对武都红芪种子萌发及幼苗生理特性的影响

Effects of olive oil processing liquid wastes on seed germination and seedling physioloyical characteristics of Radix Xedysari of Gansu

以甘肃道地中药材红芪(Hedysarum polybotrys Hand.-Mazz.)为对象,通过室内培养试验,研究不同浓度的橄榄油加工液体废弃物对红芪种子萌发、幼苗生长以及生理特性的影响,为探究橄榄油加工液体废弃物污染对红芪品质的影响提供依据。结果表明,低浓度的橄榄油加工液体废弃物能显著促进红芪种子萌发和幼苗生长,橄榄油加工液体废弃物浓度为20%时,萌发率和萌发指数分别为94.25%、384.25,胚根长和下胚轴长分别为1.94、0.93 cm,苗高和根长可达到5.28、2.96 cm。随着浓度的升高,在橄榄油加工液体废弃物浓度为70%时,种子萌发率和萌发指数显著降低,达到60.22%、254.11,幼苗生长受阻,苗高和根长可达到3.04、1.81 cm。叶片O_2^-·产生速率和H_2O_2含量明显增加,SOD、POD、CAT和APX活性先升后降,高浓度橄榄油加工液体废弃物处理下GSH和As A含量明显降低,幼苗叶片中脯氨酸、可溶性糖和丙二醛含量随废弃物浓度升高而升高。

A sand culture experiment was conducted to study the effects of different concentrations of olive oil processing liquid wastes on germination and seedling growth, osmotica accumulation and active oxygen metabolism of Radix Xedysari of Gansu authentic Chinese herbal medicine. The results showed that low concentration of olive oil processing liquid wastes stress made the seeds germination and seedling growth increased significantly, when the olive oil processing liquid wastes concentration was 20%, the germination rate and germination index were 94.25% and 384.25, embryonic root length and hypoeotyl length were 1.94 cm and 0.93 era, seedling height and root length can reach 5.28 cm and 2.96 era.With the concentration of 70%, the seed germination rate and germination index significantly decreased, the seed germination rate and germination index significantly reduced, reached 60.22% and 254.11, and the germination percentage decreased significantly, and seedling growth wasrestricted, reached 3.04 em and 1.81 cm, the leaf 02- production rate and H202 content increased dramatically, the activities of superoxide dismutase, peroxidase, catalase and ascorbate peroxidase decreased after an initial increase. The contents of glutathione and ascorbie acid in leaf decreased markedly. The leaf proline, soluble sugar and malonaldehyde contents had a significant increase with the increasing concentration of olive oil processing liquid wastes.