运用GT/PCL纳米纤维电纺膜在猪皮下构建组织工程化软骨的实验研究

The Construction of Tissue Engineered Cartilage by Using GT/PCL Nanofibres Membranes in Porcine Model

目的探讨运用GT/PCL电纺材料为支架,在大动物皮下构建组织工程化软骨的可行性。方法成年大白猪耳廓软骨获取种子细胞,分别以GT/PCL电纺材料(实验组)和传统PGA/PCL(对照组)支架材料构建软骨。细胞-材料复合物在体外培养3周后,回植入自体猪皮下。分别于回植前、体内培养3周时行组织学检测、湿重和力学强度检测,比较两组的软骨形成情况。结果体外培养3周后,两组均可见软骨组织形成,均可见明显未降解材料,对照组软骨基质分泌更为旺盛;体内培养3周后,实验组形成成熟的软骨组织,对照组未见明显软骨形成,基本为纤维组织所替代;实验组力学强度明显大于对照组。结论 GT/PCL电纺材料可以作为支架,在大动物皮下成功构建组织工程化软骨组织,是一种具有临床应用前景的支架材料。

Objective To investigate the possibility of the tissue engineered cartilage construction with electrospun gelatin/polycaprolactone （GT/PCL） membranes in subcutaneous of porcine model. Methods Engineered cartilage was constructed by auricular chondrocytes of porcine as seed cells with GT/PCL membranes （experimental group） and PGMPLA material （control group） as scaffold. Then the engineered cartilage was implanted subcutaneously in the abdominal wall of swine after cultured 3 weeks in vitro. The samples of the two groups were collected at two time points： 3 weeks in vitro and 3 weeks in vivo. Histological observation, wet weight and mechanical strength test were used to evaluate the chondrogenesis. Results After 3 weeks cultured in vitro, cartilage phenotype and undegraded scaffolds were observed in two groups by histological analysis. And the extracellular matrix in control group seems more uniform than the experimental group. However, After 3 weeks cultured in vivo, mature cartilage were only formed in the experimental group, and fibrous tissue substitute for cartilage was observed in the control group. In addition, the engineered cartilage in experimental group showed better mechanical strength. Conclusion GT/PCL membranes could be successfully used in the construction of tissue engineering cartilage in subcutaneous of large animal. It could be a kind of promising scaffold for cartilage tissue engineering.