黄瓜线粒体DNA指纹图谱的构建及其在杂交品种纯度鉴定中的应用

Construction of mitochondrial DNA fingerprinting on cucumber and its application in purity identification of hybrid varieties

为实现黄瓜杂交种子纯度的快速鉴定,利用分子标记技术构建‘长春密刺’等21份黄瓜品种的线粒体DNA指纹图谱。结果表明:从69对线粒体引物中,共筛选出13对在21份黄瓜品种中呈现出多态性的引物。同时,基于亲本材料的指纹图谱,利用引物mtSSR 4对‘南水3号’商品种子进行检测,分子标记鉴定纯度为95.7%,且真、假杂种编号与田间形态鉴定结果一致。黄瓜线粒体DNA指纹图谱的构建,不仅能为‘南水3号’等杂交种的纯度检测工作提供新的技术指导,还可为黄瓜品种的鉴定、遗传亲缘关系分析等提供一种新的方法。

For the purpose of cucumber hybrid seed identification rapidly, molecular markers technology was used to establish mitochondrion DNA fingerprinting for 21 cucumber accessions included ＇ Changchun Mici ＇.The results showed that out of the 69 pairs of primers, 13 pairs exhibited polymorphism among the 21 cucumber accessions were screened. Additional, using primer mtSSR 4 for purity identification of ＇Nanshui3＇, the result indicated that the purity was 95.7%, which were consistent with testing results in the field. The constructed mitochondrion DNA fingerprinting of the cucumber variety would not only provide a new technical guidance for the purity identification of the hybrid such as ＇Nanshui 3 ＇, but also provide a new method for the identification and genetie relationship analysis of cucumber varieties.