摘要
目的研究分析扬州市乙型肝炎病毒基因型和病毒载量的关系。方法将278例乙肝患者按血清病毒拷贝数103-108等级分成6组,多重PCR(multiplex PCR,m PCR)分析基因型,按不同基因型在各组中的例数进行线性趋势χ~2检验,分析病毒基因型在各组中比例随载量增加而出现的趋势改变。结果 B基因型244例,C基因型60例,混合感染B+C基因型9例,线性趋势χ~2检验存在统计学意义。结论扬州市乙型肝炎病毒感染以基因B和C型为主,未检测出A、D基因型,基因B型感染比例多于C型,随病毒载量的增高,基因B型百分比呈下降而C型呈上升趋势。
Objective To analyze the relationship between hepatitis B virus genotypes and the viral load in Yangzhou city.Methods 103-108 ranks were divided into 6 groups according to the quantity of the grades of serum virus copy in 244 patients with hepatitis B from Yangzhou,Cochran Armitage trend test was carried out for multiple PCR（multiplex PCR,m PCR） according to the quantity of cases of different genotypes in each group and the trend of change was analyzed with the increased proportion of virus genotypes in each group increasing. Results The cases of genotype B were 244 in all samples and that of genotype C were60,mixed genotype B and C were 9. Therefore Cochran Armitage trend test has statistical significance. Conclusion Hepatitis B virus infection are mainly B and C genotype in Yangzhou;A and D genotype are not tested;B genotype infection rates are higher than C genotype infection;with the increasing of viral load,percentage of B genotype is declining and C genotype is rise.
作者
唐阳
曹燕
杨立坤
肖越胜
TANG Yang CAO Yan YA NG Likun XIAO Yuesheng.(Yangzhou Center for Clinical Laboratory ,Jiangsu Yangzhou 225001, China.)
出处
《实验与检验医学》
CAS
2017年第1期36-38,59,共4页
Experimental and Laboratory Medicine
基金
江苏省中医药局科技项目。编号:LZ13199
