宜春市2011年至2012年乙型流感病毒耐药基因分析

Analysis of drug resistance gene in influenza B virus in Yichun from 2011 to 2012

目的分析宜春市2011~2012年B型流感病毒耐药基因位点,掌握其耐药情况,为临床治疗和疾病控制提供依据。方法采集宜春市流感监测医院和疑似流感疫情的流感样病例鼻咽拭子标本进行流感病毒分离,选择分离到的16株B型流感病毒进行核酸提取,采用逆转录-聚合酶链反应(RT-PCR)扩增病毒NA基因后进行基因序列测定,用DNAStar5.0,Mage3.1生物软件对测序结果进行分析处理,翻译出氨基酸序列,进行基因特性分析。结果 16株B型流感病毒NA区域核苷酸序列长度均为1140bp,编码380个氨基酸,所有毒株的NA蛋白催化活性位点和辅助位点均未发生氨基酸替换。结论16株毒株均对流感病毒神经氨酸酶抑制剂药物敏感,我们应该继续对流行株耐药情况进行检测。

Objective To understand the molecular characterization of neuraminidase（NA） genes and NA drug resistance of influenza B virus in Yi Chun from 2011 to 2012. Methods The specimens of nasopharyngeal swabs were collected from influenza like cases of monitor hospitals and influenza epidemic situation. 16 strains of influenza B virus were randomly selected for detection and extract virus RNA from the specimens. Fragments of NA genes were amplified by one-step RT-PCR and then were sequenced. The data obtained were analyzed with the software DNAStar5.0 and Mage3.1. Results The nucleotides of NA gene of 16 strains were all 1140 bp which encoded 380 amino acids. All strains had no mutation in catalytic residues and framework residues of NA gene. Conclusions All virus were sensitive to neuraminidase inhibitors,however continuous resistance surveillance is necessary for control and prevention influenza.