多发性骨髓瘤中c-myc和MDM2蛋白的表达及其临床意义

Expression of c-myc and MDM2 protein in multiple myeloma and their clinical significance

目的探讨c-myc蛋白及小鼠双微体基因(murine double minute 2,MDM2)蛋白在多发性骨髓瘤(multiple myeloma,MM)中的表达及其与临床分期的相关性。方法采用免疫组化SP三步法检测60例不同临床分期MM组织和10例非肿瘤骨髓组织中c-myc蛋白及MDM2蛋白的表达。结果 c-myc蛋白在MM及非肿瘤骨髓组织中的阳性率分别为71.7%、10.0%,MDM2蛋白在MM及非肿瘤骨髓组织中的阳性率分别为80.0%、20.0%,两者在MM及非肿瘤骨髓组织中的表达差异有统计学意义(P<0.05)。两者表达与MM的临床分期呈正相关性(P<0.05),与MM患者性别、年龄无相关性。MM中c-myc蛋白和MDM2蛋白的表达呈正相关(P<0.05)。结论 c-myc蛋白和MDM2蛋白在MM中高表达,其可能与MM的发生及临床分期有关;在MM中,c-myc蛋白和MDM2蛋白可能具有协同作用,共同促进肿瘤的发展。

Purpose To investigate the expression of c-myc and MDM2 protein in multiple myeloma （MM） and their correlation with clinical stage. Methods Immunohistochemical SP three-step method was used to detect the expression of MDM2 and c-myc protein in 60 cases of MM with different clinical stages and 10 cases of nontumorous bone marrow tissue. Results The positive rate of c-myc protein in MM and nontumorous bone marrow tissue were 71.7% and 10. 0%; the positive rate of MDM2 protein in two tissues were 80. 0% and 20. 0% , respectively. The expression difference of two proteins between MM and nontumorous bone marrow tissue was statistically significant （P 〈 0. 05 ）. The expression of c-myc and MDM2 protein was positively correlated with MM clinical ISS stage （ P 〈 0. 05 ） , but irrelevant with the age and gender. The expression of c-myc and MDM2 protein in MM was positively correlated （ P 〈 0. 05 ）. Conclusion The c-myc and MDM2 protein have a highly expression in MM, and it may he relative to the occurrence of MM and clinical stage. In the MM, c-myc and MDM2 protein may have synergetic functions and promote the development of tumor.