长链非编码RNA HOTAIR在中耳胆脂瘤中的表达

Study of long non-coding RNA HOTAIR expression in middle ear cholesteatoma

目的:探讨部分长链非编码RNA(lncRNA)在中耳胆脂瘤上皮组织与正常外耳道皮肤组织中是否存在表达差异。方法:采用实时荧光定量PCR方法,分别检测25例中耳胆脂瘤上皮组织及15例正常外耳道皮肤组织样本中6种lncRNA(HOTAIR、ANCR、TINCR、PRINS、BANCR、PICSAR)的表达情况;并对比不同中耳胆脂瘤骨质破坏程度的患者之间,其lncRNA的表达水平是否存在差异。结果:相对于正常外耳道皮肤组织,中耳胆脂瘤上皮组织中HOTAIR表达水平显著升高,且差异有统计学意义(P<0.01),其余5种lncRNA在中耳胆脂瘤与正常外耳道皮肤组织中的表达差异无统计学意义(P>0.05);而不同骨质破坏程度的胆脂瘤患者之间,其肿瘤组织HOTAIR表达水平差异无统计学意义(P>0.05)。结论:中耳胆脂瘤上皮组织中HOTAIR的表达较正常外耳道皮肤组织上调,其上调水平与胆脂瘤对周围骨质破坏程度之间无明显相关。

Objective：To discuss the different expression of some long-chain noncoding RNA between middle ear cholesteatoma epithelial tissue and normal external ear canal skin. Method：Real-time quantitative PCR was used to detect the expression of 6 kinds of lncRNA： HOTAIR, ANCR, TINCR, PRINS, BANCR, PICSAR in 25 ca- ses of cholesteatoma epithelial tissues and 15 cases of normal external auditory canal skin tissue samples, respectively. And compared the expression level of IncRNA in patients with different degree of bone destruction. Result： Expression level of HOTAIR was significantly increased in cholesteatoma epithelial tissues compared with the nor- mal external auditory canal skin tissues,and the difference is statistically significant（P〈0.01）. While there was no statistically significant difference expression of the rest of the five kinds of lncRNA between middle ear chol- esteatoma epithelial tissues and normal external ear canal skin （P〉0.05）. And there was no statistically significant difference expression of HOTAIR in patients with different degree of bone destruction（P〉0.05）. Conclusion： Expression level of HOTAIR was up-regulated in middle ear cholesteatoma epithelial tissue compared with the normal external auditory canal skin tissues, and the expression level of HOTAIR has no obvious correlation with degree of bone destruction in patients with cholesteatoma.