阻塞性睡眠呼吸暂停低通气综合征T细胞CD69的表达

CD69 expression on T cell surface in patients with obstructive sleep apnea hypopnea syndrome

目的:探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者外周血T细胞CD69表达率的检测及意义。方法:选择经PSG确诊的81例OSAHS患者,根据AHI指数分为轻、中、重度3组,每组27例;另选取27例无OSAHS的正常打鼾者为对照组。流式细胞术检测T细胞CD69表达率,分析T细胞CD69表达率与性别、年龄、BMI及PSG指标的相关性。结果:1OSAHS患者外周血T细胞CD69表达率随着鼾症程度上升逐渐增加(P<0.05);两两比较提示对照组与轻度组间T细胞CD69表达率无明显差别(t=1.649,P>0.05),其余组间比较均差异有统计学意义(P<0.05)。2OSAHS患者外周血T细胞CD69表达率与BMI、年龄、性别无相关性(P>0.05),与AHI呈正相关(P<0.01),与LSaO2呈负相关(P<0.01)。3联合手术及CPAP综合治疗的27例重度OSAHS患者AHI及外周血T细胞CD69表达率明显降低,LSaO2明显上升,差异有统计学意义(均P<0.01)。结论:外周血T细胞CD69表达率的上升可能为OSAHS并发心血管病的机制之一,T细胞CD69表达率的检测对反映OSAHS患者病情程度及评估并发心血管损害趋势具有一定的临床意义。

Objective： To investigate the detection and significance of T cell CD69 expression in peripheral blood of patients with obstructive sleep apnea hypopnea syndrome（OSAHS）. Method： According to AHI, 81 OSAHS patients diagnosed by PSG were divided into 3 groups： light, medium and heavy, with 27 cases in each group- 27 patients without OSAHS as control group. Flow cytometry was used to detect the expression rate of CD69 in T cells, to analyze the correlation between the expression rate of CD69 on T cells and the gender, age, BMI, and PSG index. Result：①The CD69 expression rate of T cells in peripheral blood of OSAHS patients with snoring degree increases gradually（P〈0.05）;Comparison between the two-shows that there was no significant difference in CD69 expression rate on T cells between the control group and the mild group（t= 1. 649,P〉0.05）; there were significant differences between the other groups（P〈0.05）. ②The CD69 expression rate of T cells in peripheral blood of OSAHS patients has no correlation with BMI, age and gender（P〉0.05） ,were positively cor- related with AHI, negatively correlated with LSaO2 （P%0.01）. @The CD69 expression rate of T cells and AHI in 27 cases of severe OSAHS patients with a comprehensive treatment has significantly reduced,LSaO2 increased sig- nificantly（P〈0.01）. Conclusion： Increased expression of CD69 in peripheral blood T cells may be one of the mech- anisms of OSAHS complicated with cardiovascular disease. Detection of CD69 expression rate in T cells for reflecting the degree of disease in patients with OSAHS,assessment of risk of cardiovascular damage,have certain clinical significance.