调中汤对脾虚肝旺型功能性消化不良幼鼠胃肠动力的影响

Study on Effect of Tiaozhong Decoction on Gastrointestinal Motility in Spleen-deficiency and Liver-hyperactive Young Rats with Functional Dyspepsia

目的:通过观察调中汤对脾虚肝旺型功能性消化不良幼鼠胃肠动力的影响,探讨调中汤对脾虚肝旺型功能性消化不良的具体作用机制。方法:将清洁级SD幼鼠36只,日龄21~28 d,体质量50~70 g,随机分为6组,每组6只:空白对照组、模型组、调中汤高剂量组、调中汤中剂量组、调中汤低剂量组、多潘立酮组(西药组)。幼鼠在购入后适应环境喂养1周,正常喂养,第2周开始,除对照组每日常规饲养不予造模外,其余组采用不规则喂养、饮用4℃冰水及夹尾刺激法造模。造模成功后,从造模后第7天开始用药,持续给药14 d。各组幼鼠在末次给药前12 h禁食(自由饮水),1 min内处死动物,同时取出胃及小肠。计算胃排空率及小肠推进速度。结果:调中汤高、中、低剂量组幼鼠胃排空率明显升高,较模型组比较(P<0.01);调中汤高剂量组能明显促进幼鼠小肠推进比,与模型组比较(P<0.01)。结论:成功建立脾虚肝旺型功能性消化不良幼鼠模型,并观察到调中汤能明显提高脾虚肝旺型功能性消化不良幼鼠胃排空率、小肠推进速度。与西药组比较,调中汤高剂量组效果更好。说明提高幼鼠胃排空率、小肠推进比是调中汤治疗脾虚肝旺型FD的作用机制之一。

Objective ： To study the impact of Tiaozhong Decoction on gastrointestinal motility in the spleen - deficien- cy and liver - hyperactive young rats with functional dyspepsia （FD）. To investigate the exact mechanism of Tiaozhong Decoction. Methods ： Thirty - six clean grade SD young rats, aged 21 - 28 d, weighing 50 - 70 g, were randomly divided into 6 groups ： the normal control group, the model control group, the high dose group of Tiaozhong Decoction, the middle dose group of Tiaozhong Decoction, the low dose group of Tiaozhong Decoction and the domperidone group. There were 6 rats in each group. These young rats were fed regularly on the 1 st week to adapt to the environment. From the 2nd week, all the rats were fed irregularly and the clip tail stimulation method was adopted, except for the the normal control group rats,to set up FD models. Medication was administered continuously for 14 days after the model was successfully estab- lished. Young rats in each group were fed nothing except water for 12 hours before medicine. Animals were killed in 1 rain, and the stomach and small intestine were taken out to calculate the rate of gastric emptying and small intestinal pro- pulsion ratio. Results： The gastric emptying rate of rats in Tiaozhong Decoction high, medium and low dose groups in- creased significantly compared with the model group＇ s（ P 〈0.01 ）. High dose group can obviously promote the rate of small intestine propulsion compared with model group （ P 〈 0. 01 ）. Conclusion： The model rat was successfully estab- lished. Tiaozhong Decoction can significantly improve gastric emptying ratio and the small intestinal propulsive ratio of the spleen -deficiency and liver- hyperactive young rats with functional dyspepsia（FD）. Increasing rate of gastric emptying and small intestinal propulsion ratio is one of the mechanisms of Tiaozhong Decoction in treating the spleen - deficiency and liverhyperactive young rats with functional dyspepsia（FD）.