消渴平合剂对STZ诱导的DN大鼠血脂及ETS-1的影响研究

Research of Xiaokeping Mixture on Blood Lipid and ETS-1 of STZ-induced Diabetic Nephropathy Rats

目的:观察消渴平合剂对链脲佐菌素(STZ)诱导的糖尿病肾病(DN)大鼠血脂及ETS-1的影响,探讨其对DN的防治作用及机制。方法:采用高糖高脂饮食联合小剂量STZ腹腔注射构建DN大鼠模型,随机分为正常组、模型组、厄贝沙坦组和消渴平合剂组各12只。16周时处死大鼠,观察各组大鼠总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)的变化,免疫组化检测肾组织ETS-1蛋白,荧光定量PCR检测ETS-1m RNA。结果:消渴平合剂可以改善DN大鼠的一般状况,与模型组比较,消渴平合剂组TC、TG、LDL-C含量显著降低(P<0.05),免疫组化显示消渴平合剂可以抑制DN大鼠肾组织ETS-1蛋白表达(P<0.05),荧光定量PCR检测显示消渴平合剂可以抑制DN大鼠肾组织ETS-1m RNA表达(P<0.05)。结论:消渴平合剂可调节糖尿病肾病血脂代谢紊乱,其防治DN机制可能与抑制肾脏ETS-1的表达有关。

Objective：To explore the mechanism of Xiaokeping Mixture on diabetic nephropathy rats（ DN rats）induced by streptozotoein （STZ） and the blood lipid and ETS - 1. Methods ： The experimental rats were induced by high fat, high sugar diet in combination with small dose of STZ. The rats were randomly divided into four groups：the normal control group（ n = 12）, the model group, the Irbesartan group and the Xiaokeping Mixture group （n = 12 ）. Rats were executed on the 16th week. The change of total cholesterol （ TC ）, triglycerides （ TG ）, low density lipoprotein （ LDL - C ） and high density lipoprotein（ HDL - C）were observed. The renal protein of ETS - 1 was detected by immunohistochemical method. The expression of ETS- lmRNA was detected by quantitative PCR. Results： The general condition of rats could be im- proved by Xiaokeping Mixture. Compared with the model group, Xiaokeping Mixture can decrease TC, TG , LDL - C and inhibit the expressions of ETS - 1 （P 〈 0. 05）. The quantitative PCR detection shows that Xiaokeping Mixture can inhibit the expressions of ETS- 1mRNA（P 〈 0. 05 ）. Conclusion： It was found that Xiaokeping Mixture could be used to treat DN. It can regulate the blood lipid of rats and the mechanism may be related to restraining the expression of ETS - 1.