中药抑制血栓形成中组织因子表达的研究概况

Research Summary on Modulating Tissue Factor of Thrombosis in Chinese Medicine

组织因子(tissue factor,TF)是生理性凝血过程与病理条件下血栓形成的启动因子,通过与凝血因子VII相互作用参与凝血过程,在血栓形成过程中起重要作用。归纳中药对血栓形成过程中组织因子的抑制作用,对于开发以TF为靶点的抗凝、抗血栓药物具有重要的意义。通过免疫组化、酶联免疫吸附法(ELISA)、反转录聚合酶链反应(RT-PCR)、发色底物法和免疫印迹法(Western blot)来检测单味中药和复方中药对血栓形成中TF蛋白、TF mRNA、蛋白激酶C(PKC)和核转录因子κB(NF-κB)表达的影响,结果表明单味中药和复方中药能降低TF的表达,机制是通过抑制TF mRNA、PKC和NF-κB表达,从而发挥抗血栓作用。

Tissue factor （TF） is starting factors of physiological coagulation process and thrombosis on pathological conditions, through the interaction with blood coagulation factor VII involved in blood clotting process. Tissue factor plays an important role in the process of thrombosis. Summarizing the inhibitory effect of traditional Chinese medicine on tissue factor in the process of thrombosis is significant for developing anticoagulant, antithrombotic drugs of target to TF. The re- view used methods of immunohistochemistry, enzyme linked immunosorbent assay （ELISA）, reverse transcription polymer- ase chain reaction （RT -PCR） ,developing substrate method and Western blot （Western blot） to detect the effect of sin- gle traditional Chinese medicine and compound traditional Chinese medicine to the expressions of TF protein,TF mRNA, protein kinase C （PKC） and nuclear transcription factor κB（ NF - κB） in thrombosis. Results show that traditional Chinese medicine can inhibit TF mRNA expression,the activation of PKC pathway and NF - κB to reduce the TF expression, thus playing an important role in anti -thrombosis.