酵母多糖对高脂诱导的胰岛素抵抗小鼠糖脂代谢的影响

Effects of Yeast Polysaccharide on Glucose and Lipid Metabolism in high-fat Induced insulin Resistance Mice

目的:探讨酵母多糖对胰岛素抵抗(IR)小鼠糖脂代谢的影响。方法:8周龄C57BL/6J雄性小鼠根据体质量及基础血液生化指标随机分为4组,正常组给予标准饲料;模型组给予高脂纯化饲料喂养;阳性药小檗碱组及酵母多糖组给予高脂饮食喂养同时预防性给药,连续16周。检测血浆葡萄糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(FFA)、低密度脂蛋白胆固醇(LDL-C)等,进行口服糖耐量试验,ELISA法测血浆胰岛素并计算胰岛素抵抗指数HOMR-IR,解剖分离小鼠的肝脏和肌肉,测定组织中脂质含量,分离部分肝脏及附睾周围脂肪组织做HE染色进行形态学观察。结果:与模型组相比,小檗碱组和酵母多糖组的GLU、TC、TG、LDL-C含量均明显下降(P<0.01,P<0.05),血浆胰岛素水平极显著下降;酵母多糖组肝脏、肌肉中的TC、TG含量均显著下降(P<0.05)。结论:酵母多糖具有降低血糖、血脂,改善胰岛素抵抗的作用,是用于防治糖、脂代谢紊乱的有效物质,具有进一步研究的价值。

Objective： To investigate the effects of yeast polysaccharide on glucose and lipid metabolism in high - fat induced insulin resistance mice. Methods ： Eight weeks old male C57BL/6J mice were randomly divided into 4 groups and fed for 16 weeks. The control group mice were fed standard diet. The model group mice were fed purified high -fat diet （HFD）. Both the berberine group and the yeast polysaceharide group were fed HFD and administered with 100 mg · kg^-1 · d^-1 yeast polysaccaride or berberine simultaneously. Measure the blood biochemical parameter. Test the glucose tolerance. The method of ELISA was used to measure insulin and calculate HOMR - IR index, dissecting the mouse liver and muscle tissues and measuring the tissular lipid level, the sections of liver and adipose tissue were stained with hema- toxylin - eosin （ H - E） for histological analysis. Results ： Compared with model group, the yeast polysaecharide group mice had a certain downward trend about plasma glucose （ GLU ） , reduced plasma triglyeeride （ TG ） , free fatty acids （ FFA）, cholesterol （TC） ,low density lipoprotein cholesterol（ LDL - C） and plasma insulin levels significantly. There was also a significant decrease of TG and TC in the liver and muscle and less lipid droplets in liver cells when compared with the control group. Conclusion： The yeast polysaccharide has the effect of improving insulin resistance, glucose and lipid metabolism and has a very potential value in the treatment with diabetes and hyperlipidaemia.