绿豆象成虫对绿豆豆荚挥发物的触角电位和行为反应

Electroantennogram and behavioral responses of Callosobruchus chinensis( Coleoptera: Bruchuidae) adults to volatiles from mung bean( Vigna radiata) pods

【目的】探究对绿豆象Callosobruchus chinensi有引诱作用的植物挥发性物质。【方法】采用触角电位(EAG)仪和Y型嗅觉仪分别测定了绿豆象成虫对绿豆Vigna radiata豆荚挥发物的触角电位和选择行为反应。【结果】EAG测定结果表明,已交配的绿豆象雌虫对23种绿豆豆荚挥发物的EAG反应值存在显著差异,对苯甲醛、2-己烯醛、己醛、辛醛和水杨醛5种醛类化合物的EAG反应值相对较高,其中对2-己烯醛的反应值最大,达20.68;且绿豆象成虫EAG反应值在1~100μg/μL浓度下,均随挥发物浓度的提高而增强,但已交配和未交配绿豆象雌雄成虫的EAG反应值无显著差异。行为试验发现,当5种醛类物质浓度达100μg/μL时,已交配绿豆象雌、雄成虫均对苯甲醛和2-己烯醛有显著正趋性,对己醛有显著负趋性,对辛醛和水杨醛无显著趋性。【结论】绿豆豆荚挥发物中2-己烯醛和苯甲醛与绿豆象偏爱选择密切相关。

【Aim】This study aims to investigate the plant volatiles attracting Callosobruchus chinensis.【Methods】The electroantennogram and behavioral responses of C. chinensis adults to volatiles from mung bean（ Vigna radiata） pods were tested by using electroantennographic（ EAG） detector and Y-tube olfactometer,respectively. 【Results】The EAG bioassay results showed that the respective EAG values of mated female adults of C. chinensis to 23 tested volatiles from V. radiata pods were different,among which the EAG values to benzaldehyde,2-hexenal,caproaldehyde,octanal and salicylaldehyde were relatively high,with the maximum value（ 20. 68） to 2-hexenal. EAG values showed that the antennal response strengthened with the increasing of concentration of the test componds,but the EAG values had no significant difference between mated and unmated adults. The results of behavioral response tests showed that the mated male and female adults of C. chinensis showed significantly positive taxis to benzaldehyde and 2-hexenal and significantly negative taxis to caproaldehyde, whereas showed no significant taxis to octanal and salicylaldehyde when the concentrations of these five compounds were 100μg /μL. 【Conclusion】Among volatiles from V. radiata pods,benzaldehyde and 2-hexenal may be themain attractants for C. chinensis.