瑞士乳杆菌LH-G51冻干菌粉生产工艺研究

Production Process of Lyophilized Powder for Lactobacillus helveticus LH-G51

目的:对瑞士乳杆菌LH-G51菌粉生产工艺进行优化。方法:通过单因素及正交试验设计,确定适合LH-G51生长的发酵培养基、冻干保护剂配方及生产工艺。结果:发酵时间、碳氮源及微量元素添加量均对LH-G51生长有明显影响。最终确定LH-G51培养基碳氮源及微量元素为葡萄糖20 g/L、大豆蛋白胨10 g/L、酵母膏15 g/L、MgSO_4·7H_2O为250 mg/L、MnSO_4·H_2O为50 mg/L、发酵时间10 h、保护剂为B。结论:根据优化工艺,LH-G51冻干菌粉活菌数可达到4.21×10^(11)CFU/g。

【Objective】To optimize the production process of freeze-dried powder of Lactobacillus helveticus LH-G51. 【Method】Optimized conditions for fermentation medium formula,freeze-drying protective agents and production processing were screened by using single factor and orthogonal experimental design. 【Result】Among different factors,carbon sources,nitrogen sources and micro-elements were the key factors during the cell culture stage. The optimal culture medium for LH-G51 was glucose 20 g / L,soy peptone 10 g / L,yeast extract 15 g / L,MgSO4·7H2O 250 mg / L,MnSO4·H2O 50 mg / L,the best protective agents was B. 【Conclusion】Under the optical conditions,the cell number of lyophilized powder of LH-G51 reached up to 4. 21 × 10^11 CFU / g.