草莓胶孢炭疽菌GPCRs超家族蛋白鉴定及生物信息学分析

Identification and bioinformatics analysis of GPCRs superfamily of Colletotrichum gloeosporioides in strawberry(Fragaria×ananassa Duchesne)

G蛋白偶联受体(G protein-coupled receptors,GPCRs)是广泛存在于生物体中的一类膜受体家族。基于粗糙脉孢菌典型GPCR蛋白氨基酸序列及其典型结构域对胶孢炭疽菌全基因组数据库进行比对分析,获得GPCRs超家族蛋白;对GPCRs超家族蛋白进行生物信息学分析。结果表明:胶孢炭疽菌中存在8个GPCR蛋白,其中碳源感应因子类有3个;类cAMP受体类有2个,信息素受体、氮源感应因子和真菌视蛋白类各1个。所有的GPCR都具有典型的7个跨膜结构域及较高比例的α-螺旋和无规则卷曲,且均不含信号肽序列。通过遗传关系比较分析发现,胶孢炭疽菌的8个GPCR蛋白聚类在一起,构成2个主要进化大分支和5个小分支,该聚类与其基于保守结构域的归属类别一致。本研究为进一步探究草莓胶孢炭疽菌GPCRs超家族成员的功能奠定了基础。

G protein-coupled receptors（GPCRs） are transmembrane receptors which extensively exist in all kinds of organisms. The study obtained the GPCR proteins and their genomic locations by using the GPCR protein amino acids sequences of Neurospora crassa and their typical domain to search the genome database of C. gloeosporioides. GPCR protein amino acid sequence of C. gloeosporioides were then analyzed and predicted by the bioinformatics tools. The results showed that there were 8 GPCR proteins in C. gloeosporioides. Among them, three GPCRs belonged to carbon sensors ,2 GPCRs were classified as cyclic adenosine monophosphate （cAMP） receptor-like protein. The remaining 3 GPCRs were 1 pheromone receptor, 1 putative nitrogen sensor and I opsin, respectively. All members consisted of seven typical transmembrane domains and high proportion of alpha helix and random curl but no signal peptide in these GPCR proteins. The results of phylogenetic trees showed that 8 GPCRs were clustered together and formed 2 major clades and 5 small branches. These clustering results were in accordance with the classification based on the domain prediction. This study laid the foundation for the further study on the function of GPCRs superfamily of C. gloeosporioides.