摘要
秀水134-Bt是以‘秀水134'为受体材料,通过根癌农杆菌介导的遗传转化方法导入抗虫基因cry1Ac1获得的转基因纯合品系,本研究对抗虫基因水稻秀水134-Bt与其原亲本‘秀水134'稻米的品质性状、理化特性及支链淀粉链长分布进行了比较分析。结果发现:转基因水稻秀水134-Bt与非转基因亲本‘秀水134'非常相似,稻米主要品质性状包括外观品质、碾磨品质、直链淀粉含量、胶稠度、淀粉黏滞性及支链淀粉的链长分布等食用品质方面基本相同,说明在水稻遗传转化操作过程中,TDNA插入并未显著改变原亲本稻米的主要品质特性。
Xiushui 134-Bt was a transgenic homozygous line developed through inserting the insect-resistantgene crylAcl into keng rice ＇Xiushui 134＇ by agrobacterium mediated transformation. The rice qualitycharacters, starch physicochemical properties and amylopectin chain length distribution were comparativelyanalyzed between it and its non-transgenic parent ＇ Xiushui 134＇. It was found that Xiushui 134-Bt and ＇ Xiushui134＇ was very similar and basically the same in terms of apparent quality,milling quality, amylose content,gelconsistency, starch viscosity and amylopectin chain length distribution, indicating that the insertion of T-DNA didnot significantly change the parent＇ s main quality characters.
作者
杨瑞芳
白建江
方军
曾威
李刚燮
朴钟泽
YANG Rui-fang BAI Jian-jiang FANG Jun Zeng Wei LI Gang-xie PIAO Zhong-ze(Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China National Academy of Agricultural Science, Suwon City 441-857, South Korea)
出处
《上海农业学报》
CSCD
2017年第1期31-34,共4页
Acta Agriculturae Shanghai
基金
中韩国际合作项目(PJ01125703)
上海市青年科技启明星计划(16QB1402500)
沪农科种字(2016)第1-3号
沪农青字(2016)第1-3号
关键词
转基因稻
秀水134-Bt
品质性状
淀粉黏滞性
链长分布
Transgenic rice
Xiushui 134-Bt
Quality character
Starch viscosity
Chain length distribution
