黏附分子VCAM-1和ICAM-1在子宫内膜异位症中的表达及意义

Expression of Vascular Cell Adhesion Molecule 1 and Intercellular Adhesion Molecule 1 in Endometrium and Serum in Endometriosis

目的:探讨血管细胞黏附分子(VCAM-1)和细胞间黏附分子(ICAM-1)在子宫内膜异位症(EMT)发病中的作用及意义。方法:采用荧光定量聚合酶链反应技术(Real-Time PCR)和免疫印迹法(Western-Blot)分别检测EMT(15例)患者异位内膜、在位内膜和对照组(15例)子宫内膜中VCAM-1、ICAM-1mRNA及蛋白的表达情况。采用双抗夹心酶联免疫吸附实验(ELISA)检测EMT组(44例)与对照组(28例)中可溶性VCAM-1(s VCAM-1)和可溶性ICAM-1(s ICAM-1)的水平,并对VCAM-1、ICAM-1在不同组中的mRNA、蛋白及血清中的表达行相关性分析。结果:1EMT异位内膜组VCAM-1、ICAM-1mRNA的表达均明显高于在位内膜组及对照组(P<0.01);在位内膜组VCAM-1、ICAM-1mRNA的表达与对照组差异无统计学意义(P>0.05)。2EMT异位内膜组VCAM-1、ICAM-1蛋白的表达明显高于在位内膜组及对照组(P<0.01),在位内膜组VCAM-1蛋白表达高于对照组(P<0.01);EMT在位内膜组ICAM-1蛋白的表达与对照组比较差异无统计学意义(P>0.05)。3EMT患者血清中s VCAM-1和s ICAM-1水平均明显高于对照组(P<0.01),Ⅲ、Ⅳ期患者血清中s ICAM-1水平较Ⅰ、Ⅱ期患者明显升高(P<0.01)。4EMT异位内膜组组织中VCAM-1与ICAM-1在mRNA、蛋白表达及血清中的水平均无相关性(P>0.05)。结论:异位内膜的VCAM-1和ICAM-1的表达异常可能在EMT的发生发展中具有重要作用,但ICAM-1与VCAM-1在EMT中可能具有各自的信号通路及蛋白表达的途径。

Objective：To study the expressions of Vascular Cell Adhesion Molecule 1 （VCAM-1） and Intercellular Adhesion Molecule 1 （ ICAM-1 ） in patients with endometriosis and to explore its effect on the onset of endometriosis. Methods：VCAM-1 mRNA and ICAM-1 mRNA were detected by real-time PCR method in 15 specimens of eutopic endometrium and 15 specimens of ectopic endometrium from patients with endometriosis（ EMT group） and 15 specimens of endometrium from patients without endometriosis（control group）. VCAM-1 and ICAM-1 protein were detected by Western-Blot method. Soluble VCAM-1 and soluble ICAM-1 in 44 serum of patients with endometriosis（ EMT group） and 28 serum of patients without endometriosis（control group） were tested by ELISA. Meanwhile the correlation of expressions of VCAM-1 and ICAM-1 were analyzed in different groups. Results： ①The expressions of VCAM-1 mRNA and ICAM-1 mRNA in ectopic endometrium were significantly higher than those of eutopic endometrium and the controls（ P 〈0.01 ）. There was no significant different mRNA expression between eutopic group and control group（P〉0.05）. ②The protein expressions of VCAM-1 and ICAM-1 in eutopic group were significantly higher than those of ectopic group and the control group（ P 〈0.01 ）. The protein expression of VCAM-1 in eutopic group were higher than those in the controls group（ P 〈0. 01 ）. There was no significant differ-ent protein expression of I CAM-1 between eutopic group and the controls group（ P 〉0. 05）. ③The concentration of sVCAM-1 and slCAM-1 in serum from patients with EMT were significantly higher than those in the control group（P〈0.01 ）. The concentration of slCAM-1 in serum from patients with EMT in stage Ⅲ - Ⅳ were significantly higher than those in stage Ⅰ- Ⅱ （ P 〈 0.01 ）. ④There was no significant correlation in the expression of mRNA,protein and serum between VCAM 1 and ICAM-1 in ectopic group and eutopic group（P〉0.05）. Conclusions.The abnormal expressions of VCAM-1 and ICAM-1 in ectopic endometrium may play an important role in the development of endometriosis. The signaling pathways and protein expression way may be different between VCAM 1 and ICAM 1 in endometriosis.