改良大鼠颈静脉采血法

An improved method of blood collection from jugular vein in rats

目的改进大鼠颈静脉采血法,以实现实验大鼠高质量、可循环采血,满足特殊实验要求。方法实验大鼠腹腔麻醉后,采取特殊固定方法,充分暴露颈静脉,使用一次性真空采血管进行采血。结果此法创伤性极低,本实验对60只大鼠进行间隔14 d的采血,一次采血1.8~2.5 m L,成功率在95%以上,对大鼠正常活动无明显影响。真空采血法保障了血清质量,血液检测与分析结果的准确性明显提高。结论在动物福利原则基础上,改进后的大鼠颈静脉采血法具有操作简捷、创伤小、采血量大、血液标本质量高、可循环采血等优点,保障了特殊实验的有效进行。

Objective To improve the method of blood collection from external jugular vein in rats, so as to repeatedly obtain blood samples of high quality and to meet the special requirements of the experiment. Methods The experimental rats were anesthetized by intraperiton.Meal anesthesia, and the jugular vein was fully exposed with special fixed method. Whereafter, the blood samples were collected by disposable vacuum blood vessels. Results This method had little traumatic effect. The blood samples were collected from 60 rats at an interval of 14 days with 1.8 to 2.5 mL each time. The success rate was above 95% ,and the normal activities of the rats were not apparently affeted. This vacuum blood collection method guaranteed the quality of serum, and the veracity of blood detection and analysis were also significantly improved. Conclusion On the basis of the principle of animal welfare,the improved rat jugular vein blood collection method has the advantages of simple operation,less trauma,large blood collection,high blood sample quality and so on, which can ensure the effect of the special experiment.