摘要
以20个辽宁省主栽香菇菌株为试材,采用ISSR分子标记方法,研究了20个菌株的遗传差异性,并采用NTSYS-PCR软件进行聚类分析。结果表明:从30个随机引物中筛选出9个ISSR备用引物,并用这9个引物对供试香菇菌株基因组DNA进行了ISSR-PCR扩增,共扩增出72条清晰条带,其中多态性条带占83.3%。通过聚类分析发现,异化系数在0.36为阈值时,20个菌株聚为4个组群。
Twenty Lentinula edodes strains mainly cultivated in Liaoning were used as test materials,the genetic diversity and cluster analysis were studied by ISSR technique and NTSYS-PCR software,respectively.The results showed that 9ISSR primers were selected from 30 random primers and 72 clear bands were identified by the ISSR-PCR amplification including 83.3% polymorphic bands.Clustering analysis found that 20 strains were gathered into four groups when the dissimilarity coefficient was 0.36.
出处
《北方园艺》
CAS
北大核心
2017年第5期82-85,共4页
Northern Horticulture
基金
辽宁省农业领域青年科技创新人才培养资助项目(2015026)
