摘要
以包括22份野生资源的5个耧斗菜物种为试材,采用AFLP分子标记方法,分析了中国耧斗菜野生资源的遗传变异和遗传多样性,为系统鉴定耧斗菜野生资源奠定基础。结果表明:10对引物共扩增出293个多态性位点,多态性位点比率为62.5%。Shannon多样性和He值分析结果表明,5个物种的遗传多样性大小顺序为无距耧斗菜>耧斗菜>尖萼耧斗菜>直距耧斗菜>华北耧斗菜。遗传距离分析结果表明,耧斗菜与尖萼耧斗菜遗传距离最小,尖萼耧斗菜与直距耧斗菜遗传距离最大。
In order to systematic identify genetic diversity of wild resources of Aquilegiain China,five species of Aquilegiaincluding 22 wild resources were investigated by AFLP markers method.The results showed that a total of 293 polymorphic fragments were produced by 10 AFLP primers,which accounted for 62.5%of the total fragments.The genetic diversity by Shannon index and He index indicated that the highest genetic diversity species was A.ecalcarata,and followed by gradual decrease as A.viridiflora,A.oxysepala,A.rockii,and A.yabeana.The genetic distance analysis indicated that A.oxysepalaand A.viridiflorashowed the shortest genetic distance,whereas A.oxysepalaand A.rockii showed the longest distance.
出处
《北方园艺》
CAS
北大核心
2017年第5期85-88,共4页
Northern Horticulture
基金
国家自然科学基金资助项目(31400131
81603223)
