DACH1在舌鳞状细胞癌及舌不典型增生中的表达及意义

Expression and significance of human Dachshund homolog 1 in tongue squamous cell carcinoma and tongue atypical hyperplasia

目的 :探讨细胞命运决定因子(Dachshund homolog 1,DACH1)蛋白在舌鳞状细胞癌发生、发展及预后中的作用。方法:应用免疫组织化学方法检测51例舌鳞状细胞癌及其对应癌周正常组织、25例舌不典型增生组织中DACH1的表达。采用SPSS16.0软件包进行统计学处理。结果 :51例舌鳞状细胞癌中,36例(70.6%)DACH1表达显著低于癌周正常组织(P<0.05);DACH1低表达与肿瘤分化差、临床分期晚及淋巴结转移相关;DACH1在舌不典型增生中的表达显著低于舌鳞癌组织(P<0.05)。单因素生存分析显示,DACH1高表达的舌癌患者,总体生存率显著高于低表达组(P<0.05)。结论:DACH1表达降低可能与舌鳞状细胞癌的发生、发展及不良预后有关,可能有助于舌癌前病变的辅助判断,并为肿瘤治疗提供潜在靶点。

PURPOSE： To investigate the expression and role of human Dachshund homolog1（DACH1）in the development and prognosis of tongue squamous cell carcinoma（TSCC）. METHODS： The expression of DACH1 was detected immunohistochemistrically in 51 samples of paraffin-embedded TSCC, paired adjacent tissues and 25 samples of atypical hyperplasia tissues of the tongue. Statistical analysis was performed using SPSS 16.0 software package. RESULTS： The results showed that 36 out of 51 TSCCs （70.6%） expressed lower levels of DACH1 compared with the paired adjacent tissues. Moreover, there was significant differences in expression of DACH1 between TSCC and paired adjacent tissues （P〈0.05）, and lower expression was associated with poor differentiation of tumors, advanced clinical stage and lymph node metastasis（P〈0.05）.In addition, the expression level of DACH1 in atypical hyperplasia tissues of tongue was also significantly lower than in tumors（P〈0.05）. Univariate survival analysis showed that the overall survival rate of patients with high expression of DACH1 was significantly higher than those with low expression of DACH1 （P〈0.05）. CONCLUSIONS： The decreased expression of DACH1 may be related to occurrence, development and poor prognosis of TSCC. It may contribute to making diagnosis for precancerous lesions in the tongue, and provide a potential effective therapeutic target for TSCC.