广西金樱子HPLC指纹图谱

HPLC Fingerprint of Rosae Laevigatae Fructus in Guangxi

目的:建立广西产金樱子的HPLC指纹图谱,并进行聚类分析和主成分分析,为评价其质量提供依据。方法:采用十八烷基硅烷键合硅胶为固定相,以甲醇-0.2%磷酸水溶液作为流动相,进行梯度洗脱,柱温25℃,流速0.8 m L·min-1,检测波长255 nm,进样量10μL。采用中药色谱指纹图谱相似度评价系统2012.1版软件对10批样品进行共有峰确认及相似度评价。运用SPSS 22.0统计学软件对HPLC指纹图谱进行聚类分析和主成分分析。结果:色谱评价软件共标定出共有色谱峰13个,从中选择了10个稳定性好,吸收峰强,特征明显的色谱峰作为金樱子指纹图谱的共有峰。用对照品指认了其中的3个化学成分,分别为儿茶素、橙皮苷、芦丁。10批金樱子样品指纹图谱的相似度均>0.96。通过聚类分析,将不同产地的10批样品分为3类,并且2个主成分的累计方差贡献率为82.98%。结论:该研究能为广西金樱子质量标准的提高提供数据支持,并为其品质与开发利用提供评价依据。

Objective： To establish the HPLC fingerprint of Rosae Laevigatae Fructus from Guangxi and make cluster analysis and principal component analysis in order to provide a basis for its quality control. Method： ODS column was used with methanol-0. 2% phosphoric acid solution as the mobile phase for gradient elution. The column temperature was 25 ℃ ; the flow rate of the mobile phase was 0. 8 mL·min ^-1 ; the detection wavelength was 255 rim, and the injection volume was 10 μL. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine （2012.1 version） was used to confirm the common peaks in 10 batches of samples and evaluate the similarity. SPSS 22.0 statistical software was used to make cluster analysis and principal component analysis for the HPLC fingerprint. Result： A total of thirteen common chromatographic peaks were identified in the software of chromatographical evaluation, and ten peaks of them with good stability, strong absorption peak, and obvious characteristics as common peaks. By comparing with control products, three chemical components were identified as catechin, hesperidin and rutin. The fingerprint similarity was greater than 0.96 for 10 batches of Rosae Laevigatae Fructus samples. 10 batches of samples from different areas were classified into three groups based on cluster analysis, and the accumulative variance contribution was 82. 98% for two principal components. Conclusion： The study can provide data support for improving quality standards of Rosae Laevigatae Fructus from Guangxi, providing