茵陈蒿汤复方配方颗粒、单味配方颗粒和传统汤剂对四氯化碳致小鼠肝损伤模型的比较

Comparison of Yinchenhao Tang Compound Decoction Dispensing Granules,Single Dispensing Granules and Traditional Decoction on Carbon Tetrachloride Induced Mice Liver Injury

目的:比较茵陈蒿汤复方合煎配方颗粒(合煎),单味分煎配方颗粒(分煎)和传统煎煮汤剂对四氯化碳(CCl4)造成小鼠肝损伤模型的影响。方法:90只小鼠随机分为空白组、模型组、联苯双酯滴丸组、合煎高、中、低剂量组(17.16,8.58,4.29 g·kg^(-1)),分煎高、中剂量组(17.16,8.58 g·kg^(-1)),传统汤剂组(4.29 g·k^(-1)),共9组。除空白组和模型组每日灌胃蒸馏水,其余7组预防性给药7 d。第7天灌胃结束后,除空白组外,其余各组腹腔注射0.05%CCl4,建立急性肝损伤模型。18 h后,眼眶取血测定各组小鼠血清丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),总蛋白(TP),白蛋白(ALB)和总胆红素(TBIL)活性。取小鼠肝脏做苏木素-伊红(HE)染色观察肝脏病理组织学变化,并取肝脏匀浆测定超氧化物歧化酶(SOD),丙二醛(MDA)水平,简单对比3种不同方法制备茵陈蒿汤中主要有效成分的变化。结果:合煎高、中、低剂量,分煎高、中剂量、传统汤剂均能降低CCl_4致肝损伤程度。合煎高剂量组效果优于高、中剂量组和传统汤剂组(P<0.05,P<0.01)。结论:茵陈蒿汤对CCl_4致小鼠急性肝损具保护作用,合煎配方颗粒可能由于合煎过程中的各有效成分溶出率不同,效果较单味分煎配方颗粒好;与传统汤剂比较,由于煎煮工艺优化有效成分含量更高,效果更好。其保护肝脏机制可能与清除自由基,抑制脂质过氧化有关。

Objective： To compare the effects of Yinchenhao Tang compound decoction dispensing granules, single dispensing granules and traditional decoction on carbon tetrachloride induced liver injury in mice. Method： Ninety mice were randomly divided into blank group, model group, positive group, Yinchenhao Tang compound decoction dispensing granules high, medium and low dose groups （ 17. 16, 8.58 and 4. 29 g·kg^-1 ）, single dispensing granules high and medium dose groups （17. 16 and 8.58 g ·kg^-1）, and traditional decoction group （4. 29 g·kg^-1）. The mice in blank group and model group received water by intragastric administration everyday, and the mice in other seven groups were treated with prophylactic administration for 7 days. After intragastric administration at 7th day, all the groups except blank group received 0.05% carbon tetrachloride by intraperitoneal injection to establish acute liver injury models. The activities or contents of serum alanine aminotransferase （ALT）, aspartate aminotransferase （ AST）, total protein （ TP）, albumin （ ALB ） and total bilirubin （TBIL） via eye socket were detected after 18 hours. Mice livers were taken for HE staining to observe the histopathological changes; liver homogenate was taken to test superoxide dismutase （SOD） and malondialdehyde （MDA） contents. The changes of main active ingredients among three different preparation methods were simply compared. Result： All of Yinchenhao Tang compound decoction dispensing granules, single dispensing granules and traditional decoction can reduce the degree of CC14-induced liver injury; while the effects of Yinchenhao Tang compound decoction dispensing granules high dose group were superior to the single dispensing granules and traditional decoction groups （P 〈 0. 05, P 〈 O. 01）. Conclusion： Yinchenhao Tang can protect the carbon tetrachloride induced liver injury. Effect of compound decoction was better than single dispensing granules possibly due to the different active principle dissolution rate in combined frying process. As compared with traditional decoction, the compound decoction had better effect possibly due to higher effective composition content with optimization process. Its mechanism for protecting liver may be related to scavenging free radicals and inhibiting lipid peroxidation.