硬毛粗盖孔菌预处理对玉米秸秆酶水解及组分变化的影响

Effect of biological pretreatment with Funalia trogii on enzymatic hydrolysis and chemical components of corn stover

以硬毛粗盖孔菌(Funalia trogii)为研究对象,比较其在不同预处理时间的产酶特征、预处理前后玉米秸秆酶解产糖量及其组分变化。结果表明,硬毛粗盖孔菌对木质素的降解以漆酶和锰过氧化物酶的协同作用为主导,漆酶和锰过氧化物酶活最高分别为341 IU/g和33 IU/g。经生物预处理14 d后的玉米秸秆酶解产糖量可达到350.74 mg/g秸秆,较原料提升184%。玉米秸秆组分的变化与其酶解增效密切相关,F.trogii预处理14 d后木质素和半纤维素含量分别降低了33.99%和36.61%,而纤维素仅降解8.77%,木质素和半纤维素的选择性降解,可显著降低玉米秸秆酶解抗性屏障,提升其酶解糖化效率。硬毛粗盖孔菌预处理玉米秸秆可实现木质纤维素的高效转化,缩短预处理时间,降低处理成本。

The enzyme-producing properties, sugar production of corn stover hydrolysis and the changes of corn stover components were investigated by biological pretreatment using Funalia trogii before and after the pretreatment. Results showed that F. trogii on lignin degradation was mainly by the synergistic effect of laccase and manganese peroxidase. The maximum enzymatic activities of laccase and manganese peroxidase were 341 IU/g and 33 IU/g, respectively. After biological pretreatrnent for 14 d, the maximum reducing sugar yield was 350.74 mg/g corn stover, which increased by 184% compared to raw material. The components of corn stover were closely related to the efficiency of hydrolysis. Lignin and hemicellulose were degraded by 33.99% and 36.61%, respectively. Thus, cellulose was degraded by 8.77% after 14 d biological pretreatment, which showed that F. trogii could selectively degrade lignin and semi-cellulose, thus decreased the resistance barrier of corn stover hdrolysis, and improved the yield of reducing sugar. Biological pretreatment using F. trogii could greatly increase the efficient conversion oflignocellulose, shorten pretreatment time and decreased treatment cost.