MoS_2中空纳米球的制备及在超灵敏microRNA电化学生物传感器中的应用

Preparation of MoS_2 Hollow Nanospheres and Application in Ultrasensitive Electrochemical Biosensing Platform for Micro-RNA Detection

采用模板法制备了二硫化钼中空球纳米材料,利用扫描电子电子显微镜、X射线衍射仪和Raman光谱仪对材料的形貌和结构进行表征.将适配体固定在金纳米粒子和二硫化钼共同修饰的电极上构建了一种新型的微小核糖核酸(microRNA)电化学生物传感器,采用循环伏安、微分脉冲伏安和电化学阻抗等技术对构筑的传感器进行表征.结果表明,microRNA浓度在1.0×10^(-10)~1.0×10^(-16)mol/L范围内峰电流(I)与microRNA浓度的负对数(-lgc)呈良好的线性,目标miRNA的检出限为0.55×10^(-16)mol/L.构建出的传感器具备选择性好、灵敏度高、稳定性强等特性,具有广阔的应用前景.

Molybdenum disulfide hollow sphere nanomaterial was synthesized through template-assisted method, the morphological structure was characterized by scanning electron microscopy （SEM）, X-ray diffraction（XRD） and Raman spectroscopy. A novel microRNA electrochemical biosensor was constructed by immobilizing of aptamer on the electrode that was modified with Au nanoparticles and MoS2. The electrochemi- cal behavior of the modified electrode was tested by cyclic vohammograms, differential pulse vohammetric and electrochemical impedance. The results indicate that the as-prepared biosensor shows a good linear relationship between the current variation and logarithm of the miRNA concentration ranging from 1.0 × 10^-10 mol/L to1.0×10^-16 mol/L. The detection limit was estimated to be 0. 055×10^-16 mol/L. The constructed sensor shows good selectivity, high sensitivity, strong stability characteristics and has a broad application prospect.