超高效液相色谱法分析环境样品中的酚菌酮

Analysis of Fenjuntong in Environmental Samples by UPLC

[目的]采用超高效液相色谱法,建立酚菌酮在环境样品中的残留检测方法。[方法]使用ACQUITY UPLCR BEHC_(18)柱,以乙腈-水(体积比60∶40)为流动相,在UV 252.0 nm下外标法定量分析。[结果]采用丙酮提取、二氯甲烷萃取的方法,土壤和沉积物中回收率分别为82.0%~98.0%、81.0%~89.0%,相对标准偏差为0.67%~5.34%、3.54%~4.77%。采样乙腈提取,冷冻净化的方法,斑马鱼体中的回收率为74.0%~87.0%,相对标准偏差为3.4%~3.9%。[结论]该方法分析速度快、灵敏度高、重现性好,适用于酚菌酮的环境样品中的快速检测。

[Aims] A method for determination of Fenjuntong in environmental samples by UPLC was developed. [Methods] The analysis was with the mixture solution of acetonitrile and water （60：40, by vol） as mobile phase, BEHC18 column as fix phase at 252.0 nm and quantified by external standard method. [Results] The soil and sediment samples were extracted with acetone and dichloromethane. The recoveries in soil and sediment were 82.0-98.0% and 81.0-89.0%, the RSDs were 0.67-5.34% and 3.54-4.77%, respectively. The zebra fish was extracted with acetonitrile, the recovery was 74.0-87.0%, RSD was 3.4-3.9%. [Conclusions] The method is rapid and simple with high sensitivity and good reproducibility, which is suitable for determination of Fenjuntong in environmental samples.