波长切换技术同时测定加味小柴胡汤胶囊中党参炔苷等4种成分

Detection of Four Kinds of Ingredients in Modified Xiao ChaiHu Capsules by HPLC Wavelength Switching Method Simultaneously

目的:运用安捷伦1260高效液相色谱仪,建立多波长切换技术,同时测定加味小柴胡胶囊中党参炔苷、甘草苷、黄芩苷、黄芩素含量的方法。方法:采用菲罗门ODS C18柱,以乙腈(流动相A)-0.1%磷酸水溶液为流动相体系,梯度洗脱(0~40分钟,乙腈20%~45%),流速1.0 m L/min,一般波长254 nm,检测波长在每个成分出峰的时间段内切换为该成分最大吸收波长。结果:甘草苷、党参炔苷、黄芩苷、黄芩素分别在0.05~0.75、0.042~0.63、0.80~12.0、0.04~0.60μg范围内线性关系良好。相关系数分别为0.9995、0.9992、0.9999和0.9997,平均加样回收率分别为100.31%、98.92%、101.06%、100.17%,RSD%分别为1.90、1.63、2.21、1.19。结论:本研究建立的方法稳定可靠,便于操作,可以解决党参炔苷因含量较低无法检测的问题。

Objective： To establish wavelength switching method for detecting the contents of lobetyolin, liquiritin, baicalin and baicalein contained in modified XiaoChaiHu capsules by using HPLC （Agilent 1260） simultaneously. Methods： HPLC separation was performed on Phenomenex C,8 （4.6 mm×250 mm, 5 μm）, acetonitrile-0.1% phosphoric acid （0-40 min, acetonitrile 20%--45% ） as mobile phase, and the flow rate was 1.0 mL/min, the wave- length were maximum absorption wavelength according to every component respectively. Results： Liquiritin, lobetyolin, baicalin and baicalein showed good linear relationship in the range between 0.05-0.75μg, 0.042-0.63 μg, 0.80-12.0 μg and 0.04-0.60 μg respectively. Correlation coefficients were 0.9995, 0.9992, 0.9999 and 0.9997, aver-age recovery rates were 100.31%, 98.92%, 101.06% and 100.17%, RSD% were 1.90, 1.63, 2.21 and 1.19. Conclusion： The method, stable, reliable and easy to operate, could solve the problem that lobetyolin is too low to detect.