细胞治疗与组织工程方法再生涎腺的研究进展

Research progress in cell therapy and tissue engineering approach to regenerate salivary gland

目的综述细胞治疗与组织工程方法再生涎腺的研究新进展,为治疗涎腺功能障碍提供理论依据。方法广泛查阅近年有关细胞治疗与组织工程方法再生涎腺的文献,并进行总结分析。结果细胞治疗与涎腺组织工程方法均可修复涎腺功能,但三维培养的涎腺类器官在器官发生和功能表达过程中的机制,及组织特异性细胞外基质在其中的作用问题,仍有待进一步研究。结论需要进一步深入开展干细胞治疗和组织工程人工涎腺的基础研究,建立涎腺类器官的体外培养体系,开展涎腺干细胞或其他组织来源的干细胞及干细胞转基因后移植的体内效应研究。

Objective To review the research progress in cell therapy and tissue engineering approach to regenerate salivary gland so as to provide a theoretical basis for the treatment of salivary hypofunction. Methods The recent literature on cell therapy and tissue engineering for the regeneration of salivary glands was reviewed and summarized. Results It is feasible to repair the salivary function by using various stem cells to repair damaged tissue, or by establishing salivary gland tissue ex vivo for salivary gland function restoration and reconstruction. However, the mechanism of three dimensional culturing salivary organoids during organogenesis and function expressing and the potential influence of tissue specific extracellular matrix during this process should be further studied. Conclusion Basic research of cell therapy and salivary tissue engineering should be deeply developed, and a standardized culturing system should be established in vitro. In addition, it is of great significance to study the in vivo effects of salivary gland-specific cells, non salivary gland epithelial cells and transplanted gene-transfected stem cells.