猕猴桃SCoT遗传多样性分析及指纹图谱的构建

Genetic Diversity and Fingerprints with SCoT Markers in Actinidia

利用SCoT标记对32个猕猴桃品种进行了遗传多样性分析。从47个SCoT引物中筛选了11个引物进行PCR扩增,共扩增出185个条带,其中多态性条带180个,多态性比率为97.30%。各引物Nei’s基因多样性指数(H)平均为0.238 4,Shannon’s信息指数(I)平均为0.377 8。利用UPGMA构建32份猕猴桃种质资源的聚类树状图。在遗传相似系数为0.78处可将32个猕猴桃品种分为5组,聚类结果与形态学分类基本一致。利用4条引物扩增的16个多态性位点构建了32个猕猴桃品种的DNA指纹图谱,可以将32个猕猴桃品种区分并准确鉴定。

SCoT were used to analysis genetic diversity of the samples of 32 Actinidia varieties. The 11 primers were screened from 47 SCoT primers and 185 SCoT bands were obtained, including 180 polymorphic bands, with a polymorphism rate of 97.30%. The average Nei＇s gene diversity（H） and Shannon＇ s information index （I） were 0. 2384 and 0. 3778. The cluster analysis conducted with UPGMA showed that 32 Actinidia varieties were divided into 5 groups at the genetic similarity coefficient of 0.78, and the constructed phylogenetic trees based on SCoT were consistent with the morphology results. The DNA fingerprints for 32 Actinidia varieties were established with 16 sites from 4 SCoT primers, and 32 Actinidia varieties could be identified by SCoT fringerprints.