三维共培养模型下人脐静脉内皮细胞对T24膀胱癌细胞侵袭、迁移及增殖能力的影响

Influence of co-culturing human umbilical vein endothelial cells with T24 bladder cancer cells on the invasion,migration and proliferation of T24 cells

目的:研究人脐静脉内皮细胞(HUVEC)对T24膀胱癌细胞侵袭、迁移及增殖能力的影响。方法:构建HUVEC和T24膀胱癌细胞的Trans-well共培养模型(共培养组),与T24膀胱癌细胞非共培养组进行比较,分别应用Trans-well小室、Matrigel胶及CCK-8法比较两组细胞的侵袭、迁移及增殖能力。结果:侵袭实验24h后,共培养组与非共培养组的侵袭细胞数目分别为(93.22±27.35)个、(52.04±22.54)个,共培养组侵袭细胞数显著高于非共培养组(P<0.01)。迁移实验24h后,共培养组与非共培养组的迁移细胞数目分别为(183.62±62.07)个、(66.80±23.36)个,共培养组迁移细胞数显著高于非共培养组(P<0.01)。在增殖实验中,共培养组和非共培养组的吸光度(OD)值在第3、第4、第5天分别为(1.128±0.125)、(0.866±0.068)、(1.536±0.098)和(1.103±0.153)、(1.910±0.139)、(1.550±0.177)。共培养组的细胞增殖率显著高于非共培养组(P<0.01)。结论:在三维共培养条件下,HUVEC对T24膀胱癌细胞的侵袭、迁移及增殖能力均有促进作用。

Objective：To assess the effects of co-culturing human umbilical vein endothelial cells （HUVEC） with T24 bladder cancer cells on the invasion, migration and proliferation of T24 cells. Methods： We divided the study into two groups： a T24 blad- der cancer cell group （T24 group） and the HU- VEC-T24 bladder cancer cells trans-well co-culture group （co-culture group）. The cell invasion, mi- gration ability, and proliferation were compared u- sing trans-well cell invasion Matrigel assay, trans- well migration assay and CCK-8 proliferation assay.Results： After 24 hours, the number of invasive and migrated cells in the co-cuhure group were respectively 93.22±27.35 and 183.62±62.07, which were significantly higher than those in the T24 group （52.04±22.54 and 66.80±23.36） （ P〈0.01）. The CCK-8 assay showed that the OD values on day 3, 4 and 5 were respectively （1. 128±0. 125）, （0. 866±0. 068） and （1. 536±0. 098） in the co-culture group, and were （1. 103±0. 153）, （1. 910±0. 139） and （1. 550±0. 177） in the T24 group. The cell proliferation rate was markedly higher in the co-culture group than the T24 group （ P〈0. 01）. Conclusion： Co-culture of HU- VEC with T24 bladder cancer cells promotes the invasion, migration and proliferation of T24 cells.