摘要
目的:研究人脐静脉内皮细胞(HUVEC)对T24膀胱癌细胞侵袭、迁移及增殖能力的影响。方法:构建HUVEC和T24膀胱癌细胞的Trans-well共培养模型(共培养组),与T24膀胱癌细胞非共培养组进行比较,分别应用Trans-well小室、Matrigel胶及CCK-8法比较两组细胞的侵袭、迁移及增殖能力。结果:侵袭实验24h后,共培养组与非共培养组的侵袭细胞数目分别为(93.22±27.35)个、(52.04±22.54)个,共培养组侵袭细胞数显著高于非共培养组(P<0.01)。迁移实验24h后,共培养组与非共培养组的迁移细胞数目分别为(183.62±62.07)个、(66.80±23.36)个,共培养组迁移细胞数显著高于非共培养组(P<0.01)。在增殖实验中,共培养组和非共培养组的吸光度(OD)值在第3、第4、第5天分别为(1.128±0.125)、(0.866±0.068)、(1.536±0.098)和(1.103±0.153)、(1.910±0.139)、(1.550±0.177)。共培养组的细胞增殖率显著高于非共培养组(P<0.01)。结论:在三维共培养条件下,HUVEC对T24膀胱癌细胞的侵袭、迁移及增殖能力均有促进作用。
Objective:To assess the effects of co-culturing human umbilical vein endothelial cells (HUVEC) with T24 bladder cancer cells on the invasion, migration and proliferation of T24 cells. Methods: We divided the study into two groups: a T24 blad- der cancer cell group (T24 group) and the HU- VEC-T24 bladder cancer cells trans-well co-culture group (co-culture group). The cell invasion, mi- gration ability, and proliferation were compared u- sing trans-well cell invasion Matrigel assay, trans- well migration assay and CCK-8 proliferation assay.Results: After 24 hours, the number of invasive and migrated cells in the co-cuhure group were respectively 93.22±27.35 and 183.62±62.07, which were significantly higher than those in the T24 group (52.04±22.54 and 66.80±23.36) ( P〈0.01). The CCK-8 assay showed that the OD values on day 3, 4 and 5 were respectively (1. 128±0. 125), (0. 866±0. 068) and (1. 536±0. 098) in the co-culture group, and were (1. 103±0. 153), (1. 910±0. 139) and (1. 550±0. 177) in the T24 group. The cell proliferation rate was markedly higher in the co-culture group than the T24 group ( P〈0. 01). Conclusion: Co-culture of HU- VEC with T24 bladder cancer cells promotes the invasion, migration and proliferation of T24 cells.
作者
高超
王琪
刘翰楠
吴健勇
李宇虹
庞波
梁霞
唐勇
Gao Chao Wang Qi Liu Hannan Wu Jianyong Li Yuhong Pang Bo Liang Xia Tang Yong(Department of Urology Department of Experimental Research Department of Radiotherapy, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China)
出处
《广西医科大学学报》
CAS
2017年第3期337-341,共5页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.81560428
No.81360341)
广西自然科学基金资助项目(No.2011GXNSFC018020
No.2012GXNSFAA053163)
广西科学研究与技术开发计划项目(No.桂科攻1355005-3-11)
广西医疗卫生适宜技术研究与开发项目(No.S201301-06)
广西卫生厅科研基金资助项目(No.Z2011226)
关键词
三维共培养模型
T24膀胱癌细胞
人脐静脉内皮细胞
侵袭
迁移
增殖
co-culture model
T24 bladder cancer cells
human umbilical vein endothelial cells
invasion
migration
proliferation