杨树PtROP家族基因的表达分析与功能预测

Expression and Functional Analysis of ROP Gene Family in Populus

[目的]为探究ROP基因在林木中的功能。[方法]本研究以杨树为模式,在全基因组水平上对ROP基因的家族成员、基因结构、保守结构域、氨基酸序列相似性及表达模式进行了分析。[结果]显示,杨树PtROP基因家族包含13个成员,不同成员间在进化上相对保守,均存在与GTP结合与水解相关的结构域。这些基因在不同组织、不同胁迫条件下的表达具有明显差异,说明它们参与不同的生物学过程。通过构建功能基因网络(functional gene network for poplar)发现PtROP基因主要参与了信号转导过程。[结论]杨树PtROP基因家族包含13个成员参与不同的生物学过程,可能主要参与了信号转导过程。

[Objective]To explore the putative function of ROPs in forest trees. [Method]A genome-wide analysis of ROPs was performed, including the phylogeny, gene structure, conserved motifs, ROP amino sequences similarity between Populus and Arabidopsis ROP proteins and the expression patterns using Populus as a model.[Result]The results showed that there were 13 members of PtROP genes in Populus and the PtROP genes were conserved during evolution of species,all of them containing GTP binding and hydrolysis-related domains. Furthermore,the expression profiles revealed that Populus PtROP had distinct expression pattern across different tissues and different stress conditions,suggesting that PtROP genes were involved in different biological processes. In addition,the analysis on PtROP gene functional network was conducted. It is predicted that Populus PtROP genes were mainly involved in signal transduction. [Conclude]In this study,systematically bioinformatics analysis on PtROP were conducted which laid the foundation for the further exploration of poplar PtROP functions.