青斑蝶雌成虫内生殖器官形态与发育

Morphology and Development of Reproductive System of Female Adult Tirumala limniace(Cramer)

[目的]青斑蝶是重要的观赏性昆虫,准确掌握和了解其内生殖器官结构和发育状态,能够为其人工培育和利用提供理论依据。[方法]通过对1 12日龄青斑蝶雌成虫内生殖系统解剖,观察内生殖器官结构组成,并测定内生殖器官特征参数,同时根据内生殖器官各组成部分特征对雌性卵巢发育进行分级,从而系统了解青斑蝶雌成虫内生殖器官形态与发育。[结果]1)雌性青斑蝶的内生殖器官由1对卵巢、1对侧输卵管、1根中输卵管、交配囊腺和受精囊组成;2)内生殖器官发育显著受到日龄影响,卵巢管长度随着日龄增长而显著生长,至10日龄最长,卵巢小管和侧输卵管直径随日龄增长而缓慢增大;3)随着日龄的增长,卵巢中成熟卵的数量先增大后减小,未成熟卵数量始终缓慢增加;4)根据卵巢的发育特征,将卵巢发育分为乳白透明期、卵黄沉积期、成熟待产期、产卵盛期、产卵末期。[结论]雌性青斑蝶内生殖器官结构组成与其它蝴蝶类似,其发育均显著受到日龄影响,卵子发育随日龄增长而逐渐成熟。雌性青斑蝶卵巢分级将为人为调控雌蝶产卵量提供理论指导。

[Objective]Tirumala limniace is a kind of important ornamental insects. To understand the structure and developmental state of internal reproductive organs can lay a theoretical basis for artificial reproduction and utilization of T. limniace. [Method]The internal reproductive system of female T. limniace with ages from one- to twelve-day old were dissected and observed,the characteristic parameters were measured,and the ovarian development was graded according to the developmental characteristics of ovary. [Result]（ 1） The internal reproductive organs of female T. limniace contain a couple of ovaries,a couple of lateral oviducts,a common oviduct,bursa copulatrix gland and spermatheca.（ 2） The development of internal reproductive organ was influenced significantly by age. The length of ovariole increased significantly with the age increasing and reached the maximum at the10 th day,but the diameter of ovariole and lateral oviduct increased slowly with the increase of age.（ 3） With the increase of age,the amount of mature eggs in ovary increased at initial and then deduced,but the amount of immature eggs increased slowly all the time.（ 4） Based on the developmental characteristics,the ovarian development can be divided into 5 grades,i. e. milky and translucent stage,yolk deposition stage,egg maturation stage,peak stage of oviposition and final stage of oviposition. [Conclusion]The structure of internal reproductive system of female T. limni-ace is the same as other butterflies,the development of internal reproductive organs is influenced significantly by age,and the eggs mature gradually with the increase of age. Ovarian grading of T. limniace can theoretically guide the artificial control of egg laying amount.