红花多糖对大鼠肝癌肿瘤大小及癌细胞生长抑制率的影响

Effect of Safflower Polysaccharides on Tumor Size and Cell Growth Inhibition in Rats Liver Cancer

【目的】探讨红花多糖（SPS）对大鼠肝癌肿瘤大小及癌细胞生长抑制率（IR）的影响。【方法】 将50只雄性SD大鼠随机分为正常对照组、阳性对照组和SPS高（135 mg/kg）、中（45 mg/kg）、低剂量（15 mg/kg）组,每组10只。除正常对照组大鼠予以等量生理盐水灌胃外,其余的各组大鼠均经10 mg/kg二乙基亚硝胺（DEN）灌胃建立肝癌模型,其中SPS各亚组大鼠组给予不同剂量的SPS灌胃干预,阳性对照组在灌胃建立肝癌模型后不予以任何干预措施。16周后处死所有受试大鼠,比较其体重、肿瘤结节个数及肿瘤体积差异。经MTT比色实验检测不同SPS剂量（0 mg/mL、0.32 mg/mL、0.64 mg/mL、1.28 mg/mL）对大鼠肝癌CBRH-7919细胞IR的影响。【结果】 正常对照组大鼠体重均明显高于其他组大鼠（P＜0.05）;SPS各亚组大鼠体重明显高于阳性对照组大鼠,肿瘤结节数及肿瘤体积则明显低于阳性对照组大鼠,差异均有统计学意义（P＜0.05）;高剂量亚组大鼠体重明显高于中、低剂量亚组大鼠（P＜0.05）,肿瘤结节数及肿瘤体积则明显低于中、低剂量亚组大鼠,差异均有统计学意义（P＜0.05）。MTT比色实验显示：实验48 h、72 h的大鼠肝癌CBRH-7919细胞IR明显高于同剂量实验24 h的细胞IR（P＜0.05）;SPS剂量为0.64 mg/mL时大鼠肝癌CBRH-7919细胞的IR最高（P＜0.05）。【结论】 SPS对大鼠肝癌肿瘤及肝癌细胞增殖均具有抑制作用。

【Objective】To explore the inhibitory effect of safflower polysaccharides （SPS） on the proliferation of rat liver cancer cells. 【Methods】Fifty male SD rats were randomly divided into the normal control group, the positive control group and the SPS high （135mg/kg）, middle （45mg/kg） and low-dose （15mg/kg） groups, with 10 rats in each group. Rats in the normal control group were treated with gavage of normal saline, while the remaining groups were treated with gavage of 10mg/kg diethylnitrosamine （DEN） to liver establish gastric cancer models. The SPS subgroups rats were treated with gavage of different doses of SPS. After 16 weeks of intervention, all rats were sacrificed. Rat weight, tumor nodules and tumor volume were recorded. The effect of different doses of SPS （0mg/mL, 0.32mg/mL, 0.64mg/mL, 1.28mg/mL） on the growth inhibition rate （IR） of rat liver cancer CBRH-7919 cells was detected by MTT colorimetric assay. 【Results】 The rat weight in normal control group was significantly higher than those of other groups （P〈0.05）. Body weight of SPS subgroups was significantly higher than that of the positive control group, while the number of tumor nodules and tumor volume were significantly lower （P〈0.05）. The body weight of high dose group was significantly higher than that of middle and low dose groups （P〈0.05）, while the number of tumor nodules and tumor volume were significantly lower （P〈 0.05）. MTT assay showed that the inhibition rates of rat liver cancer CBRH-7919 cells at 48h and 72h after the SPS treatment were significantly higher than that of the same dose group at 24h treatment （P〈0.05）. When the dose of SPS was 0.64 mg/mL, the IR of rat liver cancer CBRH-7919 cells was the highest （P〈0.05）. 【Conclusion】SPS has inhibitory effect on liver cancer in rat and the proliferation inhibition of rat liver cancer cells in vitro as well.