基于文献计量学心肌梗死模型大鼠血清代谢组学的特点

Metabolomics characteristics in a rat model of myocardial infarction based on bibiometrics analyses

背景:研究证实,代谢组学可以从一个总体轮廓上对大鼠心肌梗死病理代谢产物进行分析,从全面总体的角度对大鼠心肌梗死机制进行观察。目的:进一步分析大鼠心肌梗死模型血清代谢组生物信息学通路。方法:从中国知网、万方数据库、维普数据库、Embase、PubM ed数据库中检索大鼠心肌梗死的实验研究。对纳入研究代谢产物进行汇总。信号通路分析采用KEGG,代谢产物分子注释、相关的酶或转运蛋白及其相关性质分析采用HMDB,代谢产物路径可视化采用metP A网络软件。结果与结论:(1)共有10篇文献纳入分析,有26个代谢产物在这些文献中重复出现,主要参与了29条代谢路径;(2)通过通路拓扑分析选出了5条代谢通路作为大鼠心肌梗死代谢通路,分别是:氨酰tR NA生物合成、甘氨酸、丝氨酸和苏氨酸代谢、缬氨酸、亮氨酸和异亮氨酸的生物合成、不饱和脂肪酸的生物合成、苯丙氨酸,酪氨酸和色氨酸生物合成;(3)大鼠心肌梗死代谢组生物信息分析显示心肌梗死发病机制涉及到糖类、脂肪、蛋白质、RNA等物质代谢及其代谢通路。

BACKGROUND： Metabonomics has been proved to analyze and observe the pathological process of rat myocardial infarction and the underlying mechanism.OBJECTIVE： To further analyze the metabolomic pathways of bioinformatics in rat models of myocardial infarction. METHODS： The experimental studies about rat myocardial infarction were retrieved from CNKI, Wan Fang, Cq Vip, Pub Med and Embase databases. The metabolic products described in the literatures were collected and summarized. Signaling pathways were analyzed using KEGG database molecular function annotation, the enzymes, translocators and their properties were analyzed by HMDB database. Metabolites pathway were visualized with Met PA. RESULTS AND CONSLUSION： A total of 26 metabolic products were identified in the included literatures and mainly participated in 29 metabolic pathways. Through topology analysis, 5 of the 10 metabolic pathways were selected and regarded as the metabolic pathways of myocardial infarction in rats, including aminoacyl-t RNA biosynthesis; glycine, serine and threonine metabolism; valine, leucine and isoleucine biosynthesis; biosynthesis of unsaturated fatty acids; phenylalanine, tyrosine and tryptophan biosynthesis. In conclusion, the bioinformatics analysis of metabolites in rats with myocardial infarction show that myocardial infarction is related to the metabolism and metabolic pathways of carbohydrates, proteins, fat and RNA.